Quantitative planar array screen of 1000 proteins uncovers novel urinary protein biomarkers of lupus nephritis.

Objective: The goal of these studies is to discover novel urinary biomarkers of lupus nephritis (LN).

Methods: Urine from systemic lupus erythematosus (SLE) patients was interrogated for 1000 proteins using a novel, quantitative planar protein microarray. Hits were validated in an independent SLE cohort with inactive, active non-renal (ANR) and active renal (AR) patients, in a cohort with concurrent renal biopsies, and in a longitudinal cohort.

IroT/MavN Is a Transmembrane Fe(II) Transporter: Metal Selectivity and Translocation Kinetics Revealed by Real-Time Transport.

In intravacuolar pathogens, iron is essential for growth and virulence. In , a putative transmembrane protein inserted on the surface of the host pathogen-containing vacuole, IroT/MavN, facilitates intravacuolar iron acquisition from the host by an unknown mechanism, bypassing the problem of Fe(III) insolubility and mobilization. We developed a platform for purification and reconstitution of IroT in artificial lipid bilayer vesicles (proteoliposomes).