IroT/MavN Is a Transmembrane Fe(II) Transporter: Metal Selectivity and Translocation Kinetics Revealed by Real-Time Transport.

In intravacuolar pathogens, iron is essential for growth and virulence. In , a putative transmembrane protein inserted on the surface of the host pathogen-containing vacuole, IroT/MavN, facilitates intravacuolar iron acquisition from the host by an unknown mechanism, bypassing the problem of Fe(III) insolubility and mobilization. We developed a platform for purification and reconstitution of IroT in artificial lipid bilayer vesicles (proteoliposomes).