Extracellular Vesicle-Packaged miR-195-5p Sensitizes Melanoma to Targeted Therapy with Kinase Inhibitors.

Management of advanced melanoma remains challenging, with most BRAF (B-Raf proto-oncogene, serine/threonine kinase)-mutated metastatic patients relapsing within a few months upon MAPK inhibitors treatment. Modulation of tumor-derived extracellular vesicle (EVs) cargo with enrichment of antitumoral molecules is a promising strategy to impair tumor progression and increase treatment response. Herein, we report that restored expression of miR-195-5p, down-regulated in melanoma favoring drug resistance, increases the release of EVs enriched in the tumor suppressor miRNAs, miR-195-5p, miR-152-3p, and miR-202-3p.

Tumor-Derived Extracellular Vesicles: Modulation of Cellular Functional Dynamics in Tumor Microenvironment and Its Clinical Implications.

Cancer can be described as a dynamic disease formed by malignant and stromal cells. The cellular interaction between these components in the tumor microenvironment (TME) dictates the development of the disease and can be mediated by extracellular vesicles secreted by tumor cells (TEVs). In this review, we summarize emerging findings about how TEVs modify important aspects of the disease like continuous tumor growth, induction of angiogenesis and metastasis establishment.

Physicochemical properties of konjac glucomannan/alginate films enriched with sugarcane vinasse intended for mulching applications.

Biodegradable films are a promising strategy to reduce the environmental impact caused by conventional plastics commonly used in agriculture. This study focused on the production and characterization of Konjac glucomannan (KGM) and alginate (ALG) based films enriched with sugarcane vinasse (VIN), a nutrient-rich wastewater generated in large volumes by the sugar-ethanol producing industries. ALG, KGM and ALG/KGM blended (50:50) films were produced by casting and treated with calcium ions (Ca) (ALG films) and a combination of Ca, alkali, and ethanol (KGM and ALG/KGM films).