Development of the First Tritiated Tetrazine: Facilitating Tritiation of Proteins.

Tetrazine (Tz)-trans-cyclooctene (TCO) ligation is an ultra-fast and highly selective reaction and it is particularly suited to label biomolecules under physiological conditions. As such, a H-Tz based synthon would have wide applications for in vitro/ex vivo assays. In this study, we developed a H-labeled Tz and characterized its potential for application to pretargeted autoradiography.