Publications by authors named "Natasha Kreder"
Article Synopsis
- Protein kinases have a common ATP-binding site, making it hard to find selective inhibitors; however, allosteric inhibitors that target less conserved areas could be more selective and effective under high ATP conditions.
- A screening method that focuses on high ATP concentrations can help identify inhibitors that bind outside the ATP pocket, which is a strategy we used with WNK kinases to find safer antihypertensive compounds.
- The research led to the discovery of several noncompetitive WNK1-4 kinase inhibitors, which were then optimized to show a specific binding mode and effectively inhibit a sodium transporter in kidney cells, aligning with the known functions of WNK kinases in electrolyte balance.
Effective drug discovery demands the availability of microgram to gram quantities of high-quality protein encoded by novel transcripts. Protein expression vectors designed for large-scale protein production often include one or more specific tags to such transcripts, to simplify the purification of the targeted protein. Optimization of the complex expression and purification process requires the evaluation of multiple expression candidate clones to identify a production-suitable construct in terms of quality and final protein yield.
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