Publications by authors named "Natalya L Nechaeva"
Article Synopsis
- Membrane fluidity is crucial for how bacteria respond to temperature changes, with specific proteins sensing alterations in membrane thickness due to cold.
- In the cyanobacterium Synechocystis, around half of the genes that respond to cold are regulated by the protein Hik33, which also influences responses to other stress factors like salt and oxidative stress.
- The study reveals that the fluidity of the photosynthetic membrane affects the oxidation state of a key molecule (quinone pool), and this change can trigger gene expression related to cold stress, suggesting a possible universal mechanism for stress response in cells.
Conjugation to penetrating cations is a general approach for intramitochondrial delivery of physiologically active compounds, supported by a high membrane potential of mitochondria having negative sign on the matrix side. By using fluorescence correlation spectroscopy, we found here that Atto520-biotin, a conjugate of a fluorescent cationic rhodamine-based dye with the membrane-impermeable vitamin biotin, accumulated in energized mitochondria in contrast to biotin-rhodamine 110. The energy-dependent uptake of Atto520-biotin by mitochondria, being slower than that of the conventional mitochondrial dye tetramethyl-rhodamine ethyl ester, was enhanced by the hydrophobic anion tetraphenylborate (TPB).
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