New Zosteropenillines and Pallidopenillines from the Seagrass-Derived Fungus KMM 4679.

Ten new decalin polyketides, zosteropenilline M (), 11--8-hydroxyzosteropenilline M (), zosteropenilline N (), 8-hydroxyzosteropenilline G (), zosteropenilline O (), zosteropenilline P (), zosteropenilline Q (), 13-dehydroxypallidopenilline A (), zosteropenilline R () and zosteropenilline S (), together with known zosteropenillines G () and J (), pallidopenilline A () and 1-acetylpallidopenilline A (), were isolated from the ethyl acetate extract of the fungus KMM 4679 associated with the seagrass . The structures of isolated compounds were established based on spectroscopic methods. The absolute configurations of zosteropenilline Q () and zosteropenilline S () were determined using a combination of the modified Mosher's method and ROESY data.

New Cyclopiane Diterpenes and Polyketide Derivatives from Marine Sediment-Derived Fungus KMM 4670 and Their Biological Activities.

Two new cyclopiane diterpenes and a new cladosporin precursor, together with four known related compounds, were isolated from the marine sediment-derived fungus KMM 4670, which was re-identified based on phylogenetic inference from ITS, , , and gene regions. The absolute stereostructures of the isolated cyclopianes were determined using modified Mosher's method and quantum chemical calculations of the ECD spectra. The isolation from the natural source of two biosynthetic precursors of cladosporin from a natural source has been reported for the first time.

Anthraquinone Derivatives and Other Aromatic Compounds from Marine Fungus KMM 4696 and Their Effects against .

New anthraquinone derivatives acruciquinones A-C (-), together with ten known metabolites, were isolated from the obligate marine fungus KMM 4696. Acruciquinone C is the first member of anthraquinone derivatives with a 6/6/5 backbone. The structures of isolated compounds were established based on NMR and MS data.

Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived Fungus sp. 1901NT-1.2.2.

The metabolic profile of the sp. 1901NT-1.2.

Neuroprotective and Antiherpetic Properties of Polyphenolic Compounds from Heartwood.

In this study, we isolated a new isoflavanostilbene maackiapicevestitol () as a mixture of two stable conformers and as well as five previously known dimeric and monomeric stilbens: piceatannol (), maackin (), scirpusin A (), maackiasine (), and maackolin () from heartwood, using column chromatography on polyamide, silicagel, and C-18. The structures of these compounds were elucidated by NMR, HR-MS, and CD techniques. Maksar obtained from heartwood and polyphenolics - possessed moderate anti-HSV-1 activity in cytopathic effect (CPE) inhibition and RT-PCR assays.

New Bioactive β-Resorcylic Acid Derivatives from the Alga-Derived Fungus KMM 4685.

Five new β-resorcylic acid derivatives, 14-hydroxyasperentin B (), β-resoantarctines A-C (, , ) and 8-dehydro-β-resoantarctine A (), together with known 14-hydroxyasperentin (5'-hydroxyasperentin) (), were isolated from the ethyl acetate extract of the fungus KMM 4685 associated with the brown alga . The structures of the compounds were elucidated by spectroscopic analyses and modified Mosher's method, and the biogenetic pathways for compounds - were proposed. For the very first time, the relative configuration of the C-14 center of a known compound was assigned via analyses of magnitudes of the vicinal coupling constants.

Cytoprotective Polyketides from Sponge-Derived Fungus .

The new polyketides lopouzanones A and B, as well as the new 1--acetyl and 2--acetyl derivatives of dendrodochol B, were isolated from the sponge-derived marine fungus strain 168CLC-57.3. Moreover, six known polyketides, gliorosein, balticolid, dendrodolide G, dihydroisocoumarine, (-)-5-methylmellein, and dendrodochol B, were identified.

Expression of membrane beta-barrel protein in E. coli at low temperatures: Structure of Yersinia pseudotuberculosis OmpF porin inclusion bodies.

The recombinant OmpF porin of Yersinia pseudotuberculosis as a model of transmembrane protein of the β-barrel structural family was used to study low growth temperature effect on the structure of the produced inclusion bodies (IBs). This porin showed a very low expression level in E. coli at a growth temperature below optimal 37 °C.

New Antibacterial Chloro-Containing Polyketides from the Alga-Derived Fungus KMM 4696.

Six new polyketides acrucipentyns A-F (-) were isolated from the alga-derived fungus KMM 4696. Their structures were established based on spectroscopic methods. The absolute configurations of acrucipentyn A was assigned by the modified Mosher's method and ROESY data analysis.

Toporosides A and B, Cyclopentenyl-Containing ω-Glycosylated Fatty Acid Amides, and Toporosides C and D from the Northwestern Pacific Marine Sponge .

Toporosides A-D (-), new ω-glycosylated fatty acid amides, were isolated from the sponge . The structures of these compounds, including absolute configurations of stereogenic centers, were established using analysis of 1D and 2D NMR, ECD, and HR mass spectra as well as chemical transformations. Toporosides A () and B () are the first lipids containing a cyclopentenyl α,β-unsaturated carbonyl moiety in the polymethylene chain.

Studies on the Structure and Properties of Membrane Phospholipase A Inclusion Bodies Formed at Low Growth Temperatures Using GFP Fusion Strategy.

The effect of cultivation temperatures (37, 26, and 18 °C) on the conformational quality of phospholipase A (PldA) in inclusion bodies (IBs) was studied using green fluorescent protein (GFP) as a folding reporter. GFP was fused to the C-terminus of PldA to form the PldA-GFP chimeric protein. It was found that the maximum level of fluorescence and expression of the chimeric protein is observed in cells grown at 18 °C, while at 37 °C no formation of fluorescently active forms of PldA-GFP occurs.

New Deoxyisoaustamide Derivatives from the Coral-Derived Fungus KMM 4689.

Seven new deoxyisoaustamide derivatives (-) together with known compounds (-) were isolated from the coral-derived fungus KMM 4689. Their structures were established using spectroscopic methods, X-ray diffraction analysis and by comparison with related known compounds. The absolute configurations of some alkaloids were determined based on CD and NOESY data as well as biogenetic considerations.

Polyphenolic Compounds from Lespedeza Bicolor Root Bark Inhibit Progression of Human Prostate Cancer Cells via Induction of Apoptosis and Cell Cycle Arrest.

From a root bark of Turch we isolated two new (7 and 8) and six previously known compounds (1-6) belonging to the group of prenylated polyphenols. Their structures were elucidated using mass spectrometry, nuclear magnetic resonance and circular dichroism spectroscopy. These natural compounds selectively inhibited human drug-resistant prostate cancer in vitro.

Biologically Active Echinulin-Related Indolediketopiperazines from the Marine Sediment-Derived Fungus .

Seven known echinulin-related indolediketopiperazine alkaloids (-) were isolated from the Vietnamese sediment-derived fungus . Using chiral HPLC, the enantiomers of cryptoechinuline B () were isolated as individual compounds for the first time. (+)-Cryptoechinuline B () exhibited neuroprotective activity in 6-OHDA-, paraquat-, and rotenone-induced in vitro models of Parkinson's disease.

Piltunines A-F from the Marine-Derived Fungus KMM 4668.

Six new carotane sesquiterpenoids piltunines A-F (-) together with known compounds (-) were isolated from the marine-derived fungus KMM 4668. Their structures were established using spectroscopic methods. The absolute configurations of - were determined based on circular dichroism (CD) and nuclear Overhauser spectroscopy (NOESY) data as well as biogenetic considerations.

Melonoside B and Melonosins A and B, Lipids Containing Multifunctionalized ω-Hydroxy Fatty Acid Amides from the Far Eastern Marine Sponge Melonanchora kobjakovae.

Melonoside B (1) and melonosins B (2) and A (3), new lipids based on polyoxygenated fatty acid amides, and known melonoside A (4) were isolated from two different collections of the marine sponge Melonanchora kobjakovae. The structures of these compounds, including their absolute configurations, were established using detailed analysis of 1D and 2D NMR, ECD, and mass spectra as well as chemical transformations. Melonosins 2 and 3 inhibit AP-1- and NF-kB-dependent transcriptional activities in JB6 Cl41 cells at noncytotoxic concentrations, demonstrating potential cancer preventive activity.

Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine Bacterium KMM 701 and Its C494N and D451A Mutants.

A novel wild-type recombinant cold-active α-d-galactosidase (α-PsGal) from the cold-adapted marine bacterium sp. KMM 701, and its mutants D451A and C494N, were studied in terms of their structural, physicochemical, and catalytic properties. Homology models of the three-dimensional α-PsGal structure, its active center, and complexes with D-galactose were constructed for identification of functionally important amino acid residues in the active site of the enzyme, using the crystal structure of the α-galactosidase from as a template.

Peculiarities of thermal denaturation of OmpF porin from Yersinia ruckeri.

Irreversible denaturation of membrane proteins in detergent solutions is similar to unfolding of water-soluble multidomain proteins and represents a complex, multistage process. Pore-forming proteins of Gram-negative bacteria are heat-modifiable proteins, i.e.

Pallidopenillines: Polyketides from the Alga-Derived Fungus Penicillium thomii Maire KMM 4675.

Eleven new polyketides, pallidopenillines 1-11, were isolated from the alga-derived fungus Penicillium thomii. The structures of these compounds were established based on spectroscopic methods. The absolute configuration of pallidopenilline A (1) as 4R, 5S, 8S, 9R, 10R, 13R was established using a combination of the modified Mosher's method, X-ray analysis, and NOESY data.

Leptin Level in Patients with Type 2 Diabetes Mellitus after Fetal Pancreatic Stem Cell Transplant.

Objectives: We aimed to determine leptin level in patients with type 2 diabetes mellitus after fetal pancreatic stem cell transplant.

Materials And Methods: We examined 14 patients (aged 43-63 years old) with type 2 diabetes mellitus, which we subsequently divided into 2 groups and examined. Group 1 comprised 8 patients who received fetal pancreatic stem cell transplant (cells were 16-18 wk gestation) performed by intravenous infusion; group 2 comprised 6 patients in the control group who were on hypoglycemic tablet therapy or insulin therapy.

New 6,6-Spiroketal from the Alga-Derived Fungus Penicillium lividum.

The new 6,6-spiroketal,sargassopenilline H (1), and known peneciraistin C (2) have been isolated from an EtOAc extract of the marine-derived fungus Penicillium lividumKMM 4663. The structure of the new metabolite was determined by HR ESIMS and 1D and 2D NMR spectroscopy. Sargassopenilline H (1) in non-cytotoxic concentration inhibited colony formation of RPMI-7951 and MDA-MB-231 cell lines.

Meroterpenoids from the alga-derived fungi Penicillium thomii Maire and Penicillium lividum Westling.

Ten new austalide meroterpenoids (1-10) were isolated from the alga-derived fungi Penicillium thomii KMM 4645 and Penicillium lividum KMM 4663. Their structures were elucidated by extensive spectroscopic analysis and by comparison with related known compounds. The absolute configurations of some of the metabolites were assigned by the modified Mosher's method and CD data.

Secondary metabolites from a marine-derived fungus Aspergillus carneus Blochwitz.

Prenylated indole alkaloids, carneamides A-C (1-3), quinazolinone derivatives, carnequinazolines A-C (5-7), aryl C-glycosides, carnemycin A, B (8, 9) and a drimane sesquiterpenoid (10), together with known compounds (11-21) were isolated from the marine-derived fungus Aspergillus carneus (Trichocomaceae) KMM 4638. The antimicrobial and cytotoxic activities of the several alkaloids were examined.