Publications by authors named "Nataly Alvarez"
(MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants.
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- The study utilizes Hain GenoType MTBDR assays to uncover that certain mutations can lead to incorrect resistance results for fluoroquinolones, impacting both wild-type and mutant probe binding.
- These mutations, while rare worldwide, are present in about 7% of multidrug-resistant tuberculosis strains in specific areas.
- This finding highlights the need for careful interpretation of assay results in TB treatment to ensure accurate resistance detection.
Detection of multidrug-resistant tuberculosis (MDR-TB), a frequent cause of treatment failure, takes 2 or more weeks to identify by culture. Rifampicin (RIF) resistance is a hallmark of MDR-TB, and detection of mutations in the rpoB gene of Mycobacterium tuberculosis using molecular beacon probes with real-time quantitative polymerase chain reaction (qPCR) is a novel approach that takes View Article and Find Full Text PDF