Application of -CF-Fluorophore BODIPY with Phenyl and Pyrazolyl Substituents for Lifetime Visualization of Lysosomes.

A bright far-red emitting unsymmetrical -CF-BODIPY fluorescent dye with phenyl and pyrazolyl substituents was synthesized by condensation of trifluoropyrrolylethanol with pyrazolyl-pyrrole, with subsequent oxidation and complexation of the formed dipyrromethane. This BODIPY dye exhibits optical absorption at λ ≈ 610-620 nm and emission at λ ≈ 640-650 nm. The BODIPY was studied on Ehrlich carcinoma cells as a lysosome-specific fluorescent dye that allows intravital staining of cell structures with subsequent real-time monitoring of changes occurring in the cells.

Evaluation of the Safety and Toxicity of the Original Copper Nanocomposite Based on Poly-N-vinylimidazole.

A new original copper nanocomposite based on poly-N-vinylimidazole was synthesized and characterized by a complex of modern physicochemical and biological methods. The low cytotoxicity of the copper nanocomposite in relation to the cultured hepatocyte cells was found. The possibility to involve the copper from the nanocomposite in the functioning of the copper-dependent enzyme systems was evaluated during the incubation of the hepatocyte culture with this nanocomposite introduced to the nutrient medium.

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity.

Novel silver/poly-1-vinyl-1,2,4-triazole nanocomposite materials-possessing antimicrobial activity against Gram-positive and Gram-negative bacteria-have been synthesized and characterized in the solid state and aqueous solution by complex of modern physical-chemical and biologic methods. TEM-monitoring has revealed the main stages of microbial cell () destruction by novel nanocomposite. The concept of direct polarized destruction of microbes by nanosilver proposed by the authors allows the relationship between physicochemical and antimicrobial properties of novel nanocomposites.