Publications by authors named "Nataliya M Samoshina"
Objective: Liposomes are promising delivery systems for pharmaceutical applications and have been used in medicine in the recent past. Preparation of liposomes requires reliable characterization and quantification of the phospholipid components for which the traditional cumbersome molybdate method is used frequently. The objective was to improve relative and absolute quantification of lipid components from liposomes.
View Article and Find Full Text PDFRecently developed lipids with the trans-2-aminocyclohexanol (TACH) moiety represent unique pH-sensitive conformational switches ("flipids") that can trigger the membrane of liposome-based drug delivery systems at lowered pH as seen in many pathological scenarios. A library of flipids with various TACH-based headgroups and hydrocarbon tails were designed, prepared, and characterized to systematically elucidate the relationship between their chemical structures and their ability to form and to trigger liposomes. Liposomes (fliposomes) consisting of a flipid, POPC and PEG-ceramide were stable at 4°C, pH 7.
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- Lipidic amphiphiles with trans-2-aminocyclohexanol (TACH) are innovative pH-sensitive "flipids" that can disrupt lipid bilayers when acidity increases.
- Researchers designed and tested various TACH-based lipids within lipoplexes that combine cationic lipid DOTAP and plasmid DNA for gene delivery.
- The TACH-lipid lipoplexes demonstrated significantly improved gene transfection efficiency compared to traditional helper lipids while maintaining similar levels of toxicity.
Article Synopsis
- Researchers created a new kind of pH-sensitive liposomes called fliposomes, designed to release their contents when exposed to lower pH levels.
- These liposomes feature unique molecules called flipids that undergo a conformational change (or "flip") in response to acidic conditions, leading to membrane disruption and rapid cargo release.
- The study demonstrated that the speed of content release can vary based on pH levels, and the design of flipids can be customized to target specific acidic environments for controlled drug delivery.
Article Synopsis
- A new pH-sensitive liposome called fliposomes has been developed, which contains unique amphiphiles (flipids) that can change shape in response to acidic conditions, leading to rapid release of the liposome's contents.
- These fliposomes were created using a specific composition (POPC, PEG-ceramide) and demonstrated stability at 4 degrees Celsius and pH 7.4 for several months.
- When loaded with specific cargo (ANTS/DPX), the fliposomes exhibited rapid content release at lower pH levels, particularly showing enhanced efficiency with certain flipids that have shorter lipid tails.
Article Synopsis
- The study focuses on creating pH-sensitive liposomes that release their cargo quickly when exposed to acidic environments, making them effective for targeted drug delivery where acidity is higher (like tumors).
- These liposomes are formed from a mixture of a specific amphiphile, a phospholipid, and a PEG-lipid conjugate, and they maintain stability for over a year at cooler temperatures while remaining effective in simulated body conditions.
- When tested, the drug-loaded liposomes showed a rapid release of their contents in low pH conditions and increased cytotoxicity against cancer cells compared to the free drug, suggesting their potential for better cancer treatment.
Glycosidases are very important enzymes involved in a variety of biochemical processes with a special importance to biotechnology, food industry, and pharmacology. Novel structurally simple inhibitors derived from cyclohexane-1,2-dicarboxylic acids were synthesized and tested against several fungal glycosidases from Aspergillus oryzae and Penicilliumcanescens. The presence of at least two carboxylic groups and one hydroxy group was essential for efficient inhibition.
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