Validation of array comparative genome hybridization for diagnosis of translocations in preimplantation human embryos.

Fluorescent in-situ hybridization (FISH) for preimplantation genetic diagnosis (PGD) of structural chromosome abnormalities has limitations, including carrier testing, inconclusive results and limited aneuploidy screening. Array comparative genome hybridization (CGH) was used in PGD cases for translocations. Unbalances could be identified if three fragments were detectable.

Pregnancy complicated by triploidy: a comparison of the three karyotypes.

We evaluated triploid pregnancy to determine whether there are clinically important differences between the three karyotypes: 69,XXX, 69,XXY, and 69,XYY. Prospectively maintained cytogenetic databases at five tertiary care centers were retrospectively reviewed over a 10-year period to identify all triploid pregnancies. Targeted ultrasounds were reviewed to identify fetal and placental findings.

The impact of LH-containing gonadotropins on diploidy rates in preimplantation embryos: long protocol stimulation.

Background: The aim of this study was to evaluate the effect of ovarian stimulation with LH-containing gonadotropins (human menopausal gonadotropin, hMG), on ploidy of human cleavage-stage-embryos.

Methods: A total of 104 women, at ages 27-43 years, undergoing one cycle of controlled ovarian hyperstimulation for IVF in combination with preimplantation genetic diagnosis, were eligible for enrollment in this retrospective, controlled cohort study. Ovarian stimulation included down-regulation with long agonist and stimulation with either recombinant FSH or hMG.

Increased efficiency of preimplantation genetic diagnosis for infertility using "no result rescue".

Objective: To improve preimplantation genetic diagnosis (PGD) accuracy by using "no result rescue" (NRR) consisting of the reanalysis of dubious results with additional probes binding to a locus different from the one previously analyzed.

Design: Prospective study of PGD cycles with and without reanalysis of inconclusive results.

Setting: PGD laboratory.

Patterns of ovarian response to gonadotropin stimulation in female carriers of balanced translocation.

Objective: To determine whether maternal balanced translocation is a risk factor for poor ovarian response to controlled ovarian hyperstimulation (COH).

Design: A retrospective analysis.

Setting: Private IVF center.

Factors associated with disposition of cryopreserved reproductive tissue.

Objective: To study patient characteristics associated with the preferences for the disposition of cryopreserved semen and embryos in the event of death or divorce.

Design: Retrospective exploratory study.

Setting: Tertiary care academic medical center.

Monozygotic twinning following assisted conception: an analysis of 81 consecutive cases.

Background: This study attempts to identify risk factors for monozygotic (MZ) twinning following assisted conception.

Methods: Eighty-one MZ twinning pregnancies that occurred over a 7-year period in our IVF/embryo transfer programme were evaluated. These were compared with 4224 non-MZ pregnancies from the same period.