Polychromatic Flow Cytometry to Identify Rare Aged Hematopoietic Stem Cell Subpopulations.

Flow cytometry enables the identification and characterization of markers present on the cell membrane as well as those that manifest intracellularly. With the increasing number of available reagents for targeting the markers of interest and evolving technology, it has become possible to detect an increasing number of markers expressed by single cells during one single analysis. This provides the possibility of investigating cell-to-cell patterns, variations, and correlations.

CD61 identifies a superior population of aged murine HSCs and is required to preserve quiescence and self-renewal.

Aging leads to a decline in function of hematopoietic stem cells (HSCs) and increases susceptibility to hematological disease. We found CD61 to be highly expressed in aged murine HSCs. Here, we investigate the role of CD61 in identifying distinct subpopulations of aged HSCs and assess how expression of CD61 affects stem cell function.

A comprehensive transcriptome signature of murine hematopoietic stem cell aging.

We surveyed 16 published and unpublished data sets to determine whether a consistent pattern of transcriptional deregulation in aging murine hematopoietic stem cells (HSC) exists. Despite substantial heterogeneity between individual studies, we uncovered a core and robust HSC aging signature. We detected increased transcriptional activation in aged HSCs, further confirmed by chromatin accessibility analysis.

FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1.

Fibroblast growth factor receptors (FGFRs) are emerging targets for directed cancer therapy. Presented here is a new FGFR1-targeting conjugate, the peptibodyF2, which employs peptibody, a fusion of peptide and the Fc fragment of human IgG as a selective targeting agent and drug carrier. Short peptide based on FGF2 sequence was used to construct a FGFR1-targeting peptibody.