Esterase Profile in (Diptera; Drosophilidae), a Non-cactophilic Species of the Group: Development Patterns and Aspects of Genetic Variability.

Esterases are a diversified group of isozymes that performs several metabolic functions in . In the group, this class of enzymes was well described in cactophilic species, existing a lack of studies considering substrate speci city and life cycle expression in the non-cactophilic species. The larvae of cactophilic species of the group develop in rotting cacti cladodes, but adults are generalists.

MISTIC-fusion proteins as antigens for high quality membrane protein antibodies.

Lack of high-quality antibodies against transmembrane proteins is a widely recognized hindrance in biomedical and cell biological research. Here we present a robust pipeline for the generation of polyclonal antibodies employing full-length membrane proteins as immunogens to overcome this "antibody bottleneck". We express transmembrane proteins fused to a MISTIC fragment that enhances expression of eukaryotic membrane proteins in E.