[Development of molecular PCR-RFLP test for identification of the epidemic strain of Y. enterocolitica bioserotype 1B/O8 circulating in Poland since 2004].

Introduction: Highly pathogenic Y. enterocolitica bioserotype 1B/O8 is considered to be an important etiological agent of yersiniosis in Poland. Infections caused by Y.

[Microbiological diagnosis of infections caused by Campylobacter jejuni and Campylobacter coli in humans].

Campylobacter jejuni and Campylobacter coli are Gram-negative, microaerophilic bacteria which are worldwide in distribution, causing a zoonotic disease in humans called campylobacteriosis. These infections are mainly caused by eating contaminated food products, most often improperly prepared poultry meat. Campylobacteriosis usually takes the form of gastroenteritis, or inflammation of the intestines, and the characteristic symptoms are watery-mucous diarrhea often with the presence of blood in stool, nausea, vomiting, abdominal pain and fever.

Seroprevalence of diphtheria toxoid IgG antibodies in children, adolescents and adults in Poland.

Background: Recommendations for diphtheria immunization are to apply an effective primary immunization in infancy and to maintain immunity throughout life. Immunity against diphtheria depends primarily on antibody to the diphtheria toxin. This study evaluated the seroprevalence of IgG diphtheria antitoxin in sera of healthy children, adolescents and adults in Poland.

[Effect of multiple freeze-thaw cycles on detection of IgA, IgG and IgM antibodies to selected bacterial antigens].

Introduction: In presented study we investigated the effect of multiple freeze-thaw cycles of human sera on the determination of IgA, IgG and IgM antibodies to selected bacterial antigens.

Methods: A panel of 15 serum samples with elevated levels of antibodies to Mycoplasma peumoniae, Yersinia enterocolitica and Salmonella spp. were used (5 positive sera for each pathogen).

[Seroprevalence of antibodies against lipopolysaccharides of Bordetella parapertussis in patients with prolonged caught in Poland].

Introduction: Bordetella parapertussis is a bacterium closely related to Bordetella pertussis, also causes a pertussis - like symptoms in humans. Because of unsatisfactory level of routine microbiological diagnosis of B. parapertussis infections in Poland most of parapertussis cases are not reported.

[Detection of antibodies to Campylobacter jejuni in pediatrics patients with Gullain-Barré syndrome using different antigen preparations].

Diagnosis of previous C. jejuni infections in GBS patients are mostly based on serological findings. However, there are not consensus what kind of antigen should be used in the serological assays.

[Comparison of usefulness of lipopolysaccharides extracted by phenol and trichloroacetic acid from Salmonella Typhimurium and Enteritidis for serodiagnosis of salmonelosis].

Salmonella Typhimurium and Salmonella Enteritidis are the two predominant serogroups, responsible for about 80% of all human cases of salmonelosis in Poland. Therefore we compared the usefulness of lipopolysaccharides antigens extracted by phenol (Westphal method) and trichloroacetic acid (Boivine method) from Salmonella Typhimurium and Enteritidis in ELISA method for the determination of antibodies. We used one home - made LPS antigen and two others commercially available antigens from SIGMA - Aldrich.

[Comparison and evaluation of the Binax EIA and Biotest EIA urinary antigen kits for detection of Legionella pneumophila antigen in urine samples].

The Binax and the Biotest urinary antigen kits for detection of L. pneumophila antigen were compared by testing of selected 67 urine samples obtained from EWGLI as reference samples in External Quality Assessment Scheme. Thirty nine were positive with the Binax kit (100% of sensitivity), and 33 were positive with the Biotest (84.

[Evaluation of usefulness of the enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to lipopolysaccharides of VTEC strains in patients suspected for Escherichia coli O104:H4 infection in Poland].

The aim of the study was to investigate the presence of antibodies to lipopolysaccharides obtained by modified Boivin's method from E. coli serotype O104:H4 and O26, O103, O111, O121, O145, O157 in sera of 7 patients with acute diarrhea, suspected in clinical investigation for infection caused by E. coli O104:H4.

[Comparison of routine serological investigations performed in three different laboratories in Poland for the diagnosis of pertussis].

We compared the 2.653 results of routine serological investigations performed in three different laboratories in Poland for the diagnosis of pertussis. One of the laboratories used the NovaLisa Bordetella pertussis kit produced by NovaTec GmbH and the two others used Bordetella pertussis ELISA kit produced by Genzyme Virotech GmbH.

[Application of enzyme linked immunosorbent assay (ELISA) for diagnosis of antibodies to lipopolysaccharide of Klebsiella rhinoscleromatis in patients with rhinoscleroma].

The ELISA were performed on polystyrene microtiter plates (Nunc, MaxiSorp) coated with LPS (2a antigen) at the final concentration of 10 microg/ml. The antigen was extracted from Klebsiella rhinoscleromatis Rh32 by the trichloroacetic acid and separated by ethanol (Boivin method). The antibodies against the LPS were detected by ELISA in serum samples collected from 65 patients suspected in clinical investigation for rhinoscleroma in Poland from 1970 to 2009.

[Microbiological diagnosis of respiratory infections caused by Legionella pneumophila].

Legionella pneumophila is an important causative agent of pneumonia in humans which is difficult to diagnose because the signs and symptoms are nonspecific and do not distinguish Legionella infection from other common causes of pneumonia. Currently, the diagnosis of Legionnaires' disease is based on phenotyping (culture, antibody detection in human sera, antigen detection in urine) and genotyping methods such as PCR (polymerase chain reaction). This review focuses on current diagnostic tests for surveillance of Legionella pneumophila infections in Poland.

[The frequency of detection of IgA, IgG and IgM antibodies to pertussis toxin in patients with respiratory tract infections in Poland].

Five hundred of results of commercial ELISA test (NovaLisa Bordetella pertussis, NovaTec) used in routine diagnosis of IgA, IgG and IgM antibodies to pertussis toxin in serum samples obtained from patients with respiratory tract infection were analyzed. The diagnostic level of IgA antibodies was observed in 54 (10.8%), IgG in 258 (51.

[Evaluation of the usefulness of selected antigens of Mycoplasma pneumoniae for the serodiagnosis of mycoplasmosis].

A panel of 33 different antigens, among them lipoproteins, glicolipids and proteins, of Mycoplasma pneumoniae used in commercial western-blotting (Virotech) were assessed for reactivity with sera of patients with mycoplasmosis and other bacterial infections of variable etiology. In addition, commercial ELISA (Virotech) with recombinant proteins as antigen and complement fixation test (CFT) with in-house prepared glicolipid-protein antigen were also assessed for comparison. The proteins with molecular weight of 170 kDa (P1) and 90 kDa (P90) were most recognized by the serum samples of patients with mycoplasmosis.

[Subclass distribution of human IgG antibodies to Mycoplasma pneumoniae in the course of mycoplasmosis].

The prevalence of human IgG1-IgG4 to sonicated antigen of M. pneumoniae in the 138 sera obtained from patients with mycoplasmosis was analysed. Antibodies of IgG1 were diagnosed in 47 (34.

[Evaluation of antigenic properties of Campylobacter jejuni and Campylobacter coli proteins in a western-immunoblot].

Campylobacter jejuni and Campylobacter coli are the most common bacterial cause for acute diarrheal illnesses in developed countries. The aim of this study was to evaluate the antigenic properties of Campylobacterjejuni and Campylobacter coli proteins in western-blot assay. Whole-cell components of Campulobacter jejuni and Campylobacter coli were separated by sodium dodecyl sulfate-polyacrylamide gel electroforesis.

[Evaluation of usefulness of commercial recomwell Campylobacter enzyme--linked immunosorbent assays for routine serodiagnosis of Campylobacter jejuni and Campylobacter coli infections].

The commercially available enzyme-linked immunosorbent assays (ELISA recomWell Campylobacter) from Mikrogen was evaluated for the diagnosis of Campylobacter jejuni and Campylobacter coli infections. Serum samples from 20 healthy controls, 44 persons with symptoms of primary Campylobacter infection and 24 serum samples from patients with Yersinia enterocolitica or Salmonella infections were tested. This ELISA assay detects IgA and IgG antibodies against three recombinant antigens of the Campylobacter jejuni and Campylobacter coli: OMP 18 (18 kDa), PEB4 (31 kDa) and P39 (39 kDa).