Mebeverine influences sodium ion transport in the distal colon.

The study was performed to check if the well-known intestinal spasmolytic effect of mebeverine is paralleled by any changes in intestinal transepithelial currents. The transepithelial potential difference related to ionic currents of the isolated rabbit distal colon wall was measured by means of Ussing's technique under control conditions and after gentle mechanical stimulation of intestinal epithelial surface by a flux from peristaltic pump and with and without of mebeverine in stimulation fluid. The transient hyperpolarization after mechanical stimulation was diminished after addition of mebeverine to the stimulation fluid when chloride transport was inhibited by bumetanide (BUME) but in the presence of amiloride (AMI), a sodium ion transport inhibitor, the drug did not influence the reaction.

The influence of ambroxol and capsaicin on the isolated rabbit bladder wall.

Unmyelinated C-fibers endings lye beneath the epithelial layer and release neuropeptides which regulate baseline transepithelial potential difference (PD) and changes of transepithelial potential difference during mechanical stimulation (dPD). Ambroxol can suppress reflexes such as the cough or the corneal reflex which are connected to stimulation of C fibre endings. The study aimed to estimate the influence of ambroxol (ABX) and capsaicin (CAPSA) on PD and dPD in isolated rabbit bladder.

Postmortem examination of transepithelial ion currents in rabbit colon and trachea in relation to temperature of storage and its importance for interlethal reactions.

Electrical phenomena resulting from transepithelial ion transport have been a subject of clinical, physiological, pharmacologic and toxicologic studies. These examinations concern mainly electric phenomena in live organisms. The changes of transepithelial ion pathways which take place postmortem have not been yet established.

Effects of halothane and isoflurane on stimulated airway transepithelial ion transport.

Volatile anesthetics are suggested to elicit depression of airway clearance. The involvement of changes in transepithelial ion transport in this inhibition has already been observed. The Ussing method was used to study the changes in mechanical stimulation evoked electrogenic ion transport in isolated rabbit tracheal wall in the presence of halothane and isoflurane.

Effects of amiloride and bumetanide on hyperpolarization after movement across the distal colon epithelium.

The aim of the paper was to define which transepithelial ion transport pathways were activated during stimulation by movement across the colonic epithelium. The experiments were performed with Ussing method on 241 specimens of isolated distal colon wall from 51 rabbits. The tissue was stimulated by a flux from peristaltic pump which caused transient hyperpolarization of the isolated tissue.

Effect of amiloride and bumetanide on transepithelial ion transport in isolated rabbit cecal and colonic wall.

Introduction: The aim of the study was to compare the effects of amiloride and bumetanide on the baseline transepithelial electrical potential difference (PD) and changes in PD during mechanical stimulation (dPD) in isolated cecal and colonic wall of rabbits.

Material/methods: The experiments were performed with a modified Ussing chamber system. Isolated tissue specimens were incubated in Ringer's solution, in amiloride and/or bumetanide, or in dimethyl sulfoxide (DMSO).