Publications by authors named "Natalia Leontyevskaya Vasilyeva"
Article Synopsis
- This study examined the antimicrobial properties of a specific strain, revealing that its culture fluid contains agents effective against various bacteria.
- The strain was found to produce outer membrane vesicles that exhibit bacteriolytic effects on both Gram-positive and Gram-negative bacteria.
- Transcriptomic analysis highlighted numerous genes related to bacteriolytic enzymes and secondary metabolites with antimicrobial potential, emphasizing the importance of isolating and researching these antimicrobial agents.
Article Synopsis
- Lysobacter capsici VKM B-2533 and Lysobacter gummosus 10.1.1 are identified as potentially valuable strains for developing new antimicrobial drugs.* -
- The whole-genome sequences of these strains were sequenced, with total lengths of 6,239,188 base pairs and 6,056,609 base pairs, respectively.* -
- Sequencing was performed using advanced technologies, specifically the Illumina and Nanopore platforms.*
A successful homologous expression system based on VKM B-2533 and the plasmid pBBR1-MCS5 was first developed for a promising bacteriolytic enzyme of this bacterium, β-lytic protease (Blp). In the expression strains, gene expression under the regulation of the GroEL(A) and T5 promoters increased by 247- and 667-fold, respectively, as compared with the wild-type strain. After the cultivation of the expression strains P- and P-, the Blp yield increased by 6.
View Article and Find Full Text PDFOuter membrane vesicles (OMVs) produced by Gram-negative bacteria constitute important factors in defining interactions with the extracellular milieu. sp. XL1 produces OMVs capable of lysing microbial cells due to the presence in their cargo of bacteriolytic protease L5 (AlpB).
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