Publications by authors named "Natalia Katarzyna Karolak"
Article Synopsis
- The removal of the mRNA 5' cap is key for gene expression regulation and leads to transcript degradation in eukaryotes.
- Unlike other eukaryotes, Kinetoplastida, such as T. brucei, use the ALPH1 enzyme for decapping instead of the typical Dcp2 enzyme, and ALPH1 forms complexes with several unique Kinetoplastida proteins.
- The structure and localization of ALPH1, particularly its C- and N-terminal regions, are crucial for its interaction with other proteins and RNA granules, indicating distinct regulatory mechanisms compared to other organisms.
CacyBP/SIP is a multifunctional protein present in various cells and tissues. However, its expression and role in the epidermis has not been explored so far. In this work, using RT-qPCR, Western blot analysis and three-dimensional (3D) organotypic cultures of HaCaT keratinocytes we show that CacyBP/SIP is present in the epidermis.
View Article and Find Full Text PDFElectrostatic energy has a significant contribution to intermolecular interaction energy, especially in biological systems. Unfortunately, precise quantum mechanics calculations are not feasible for large biological systems; hence, simpler calculation methods are required. We propose a method called UBDB+EPMM (University at Buffalo Pseudoatom DataBank + Exact Potential Multipole Moments), which shortens computational time without losing accuracy.
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