Publications by authors named "Nastaran Sadat Savar"

Article Synopsis
  • - Self-amplifying mRNA (SAM) vaccines, derived from Semliki Forest virus (SFV), have the potential to improve the efficacy and speed of vaccine development against infectious diseases and cancer.
  • - Researchers optimized protocols for preparing, packaging, and measuring the titer of SFV-PD self-amplifying mRNA, specifically focusing on its expression in HEK-293 and BHK-21 cell lines.
  • - The study demonstrated that BHK-21 cells yielded higher and more stable EGFP expression, and that ultrafiltration combined with RT-qPCR provided a rapid and accurate method for producing high quantities and assessing the quality of virus replicon particles for vaccine research.
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Article Synopsis
  • - The study aimed to create a fusion protein of two specific proteins from Leishmania major and Phlebotomus papatasi, expressing it using a self-amplifying mRNA (SAM) system packaged in viral particles.
  • - Two fusion combinations (PpSP15-LmSTI1 and LmSTI1-PpSP15) were analyzed for their structure and stability, revealing that PpSP15-LmSTI1 showed more favorable characteristics for mRNA folding and protein stability.
  • - This research is pioneering in the use of alphavirus-derived SAM and viral replicon particles (VRPs) for targeting leishmaniasis, providing promising results for future applications in recombinant protein expression and infection of
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New vaccine platforms are crucial to address complex parasitic infections such as cutaneous leishmaniasis. Self-amplifying mRNA (SAM) based vaccines represent the next generation nucleic acid-based platform. In the present study, we compared the expression levels of PpSP15-LmSTI1 fusion gene in BHK-21 cells following transfection with Semliki Forest virus (SFV)-derived SAM, SFV-derived plasmid DNA (pSFV-PD) and conventional plasmid DNA (pcDNA3.

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A newly described coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the causative agent of coronavirus disease 2019 (COVID-19), has infected over 2.3 million people, led to the death of more than 160,000 individuals and caused worldwide social and economic disruption. There are no antiviral drugs with proven clinical efficacy for the treatment of COVID-19, nor are there any vaccines that prevent infection with SARS-CoV-2, and efforts to develop drugs and vaccines are hampered by the limited knowledge of the molecular details of how SARS-CoV-2 infects cells.

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Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae.

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Background: Toll-like receptor-5 (TLR-5) is a member of TLRs family and responsible for bacterial flagellin recognition. The activation of TLR-5 with flagellin leads to initiation of signaling cascades, which in turn results in transcription of pro-inflammatory cytokines. Regarding the critical role of TLR-5 agonists and antagonists in activation of innate immune responses, an increasing number of studies have focused on their therapeutic applications in drug and vaccine design.

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The new generation of vaccines against infectious diseases is based on recombinant fusion proteins. Flagellin (FliC) of enteroaggregative Escherichia coli (EAEC) could be considered as a potent adjuvant in designing new vaccines. However, because of its large size, incorporation of this protein with a vaccine antigen might negatively influence recognition of the vaccine epitopes by the immune system.

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Enteroaggregative Escherichia coli (EAEC) is an important cause of acute and chronic diarrhea worldwide. It has been shown that flagellin (FliC), a major bacterial surface protein of EAEC, causes IL-8 release from certain epithelial cell lines via activation of TLR-5. Based on the ability of this protein to activate innate immunity, flagellin can be considered as a potent adjuvant in new vaccines and adjuvant effects of native or recombinant forms of flagellin have been demonstrated.

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