Publications by authors named "Nassim Salem"

Article Synopsis
  • In patients with acute myeloid leukemia (AML), a higher presence of Vγ9Vδ2 T cells at diagnosis is associated with better overall and relapse-free survival rates.
  • This study analyzed immunophenotypic data from 198 newly diagnosed AML patients to determine how Vγ9Vδ2 T-cell frequency impacts prognosis while adjusting for various confounding factors.
  • The findings support the importance of Vγ9Vδ2 T cells in AML prognosis and suggest potential treatment strategies that could boost these T-cell responses in patients.
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The success of immunotherapy has highlighted the critical role of the immune microenvironment in acute lymphoblastic leukemia (ALL); however, the immune landscape in ALL remains incompletely understood and most studies have focused on conventional T cells or NK cells. This study investigated the prognostic impact of circulating γδ T-cell alterations using high-dimensional analysis in a cohort of newly diagnosed adult ALL patients (10 B-ALL; 9 Philadelphia ALL; 9 T-ALL). Our analysis revealed common alterations in CD8 T cells and γδ T cells of relapsed patients, including accumulation of early stage differentiation and increased expression of BTLA and CD73.

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Herein, we report a novel approach for the design of a colorimetric aptasensor, relying on a Dye Salt Aggregation-based Colorimetric Oligonucleotide assay (DYSACO assay). This method is based on the use of an intercalating agent, Nile Blue (NB), whose aggregation capacities (and thus modification of its absorption spectrum) are drastically amplified by adding salts to the working solution. The presence of an aptamer could protect NB from such aggregation process due to its intercalation into double-stranded DNA and/or interaction with nucleobases.

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Purpose: Immune checkpoint inhibitors (ICI) have revolutionized the treatment of non-small cell lung cancer (NSCLC), but predictive biomarkers of their efficacy are imperfect. The primary objective is to evaluate circulating immune predictors of pembrolizumab efficacy in patients with advanced NSCLC.

Experimental Design: We used high-dimensional mass cytometry (CyTOF) in baseline blood samples of patients with advanced NSCLC treated with pembrolizumab.

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Natural Killer (NK) cells are potent anti-leukemic immune effectors. However, they display multiple defects in acute myeloid leukemia (AML) patients leading to reduced anti-tumor potential. Our limited understanding of the mechanisms underlying these defects hampers the development of strategies to restore NK cell potential.

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Article Synopsis
  • Natural killer (NK) cells are crucial in fighting leukemia, but their function is hindered by leukemic blasts, leading to the presence of dysfunctional NK cells in acute myeloid leukemia (AML).
  • In a study involving patients with newly diagnosed AML, researchers found a significant accumulation of a specific subset of NK cells (CD56CD16) that resemble those seen in HIV-infected individuals, indicating a potential disruption in their activation and function.
  • A higher percentage of these CD56CD16 NK cells at diagnosis correlated with poorer clinical outcomes, suggesting they might be a marker for immune evasion as AML progresses.
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NK cells are innate immune cells with inherent capabilities in both recognizing and killing cancer cells. NK cell phenotypes and functional alterations are being described with increasing precision among patients harboring various cancer types, emphasizing the critical role that NK cells play in antitumor immune responses. In addition, advances in understanding NK cell biology have improved our knowledge of such alterations, thereby expanding the potential exploitation of NK cells' anticancer capabilities.

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