Plant infection by the necrotrophic fungus Botrytis requires actin-dependent generation of high invasive turgor pressure.

The devastating pathogen Botrytis cinerea infects a broad spectrum of host plants, causing great socio-economic losses. The necrotrophic fungus rapidly kills plant cells, nourishing their wall and cellular contents. To this end, necrotrophs secrete a cocktail of cell wall degrading enzymes, phytotoxic proteins and metabolites.

Genome Comparisons between and the Closely Related Gray Mold Fungus Reveal Possible Explanations for Their Contrasting Host Ranges.

While causes gray mold on many plants, its close relative, , is host-specifically infecting predominantly faba bean plants. To explore the basis for its narrow host range, a gapless genome sequence of strain G12 (BfabG12) was generated. The BfabG12 genome encompasses 45.

Contrasting cytosolic glutathione redox dynamics under abiotic and biotic stress in barley as revealed by the biosensor Grx1-roGFP2.

Barley is a staple crop of major global importance and relatively resilient to a wide range of stress factors in the field. Transgenic reporter lines to investigate physiological parameters during stress treatments remain scarce. We generated and characterized transgenic homozygous barley lines (cv.

Multiple knockout mutants reveal a high redundancy of phytotoxic compounds contributing to necrotrophic pathogenesis of Botrytis cinerea.

Botrytis cinerea is a major plant pathogen infecting more than 1400 plant species. During invasion, the fungus rapidly kills host cells, which is believed to be supported by induction of programmed plant cell death. To comprehensively evaluate the contributions of most of the currently known plant cell death inducing proteins (CDIPs) and metabolites for necrotrophic infection, an optimized CRISPR/Cas9 protocol was established which allowed to perform serial marker-free mutagenesis to generate multiple deletion mutants lacking up to 12 CDIPs.

The Potential of Plant-Based Bioactive Compounds on Inhibition of Aflatoxin B1 Biosynthesis and Down-regulation of , and Genes.

The use of plant extracts in pre- and post-harvest disease management of agricultural crops to cope with aflatoxin B1 contamination has shown great promise due to their capability in managing toxins and safe-keeping the quality. We investigated the anti-aflatoxigenic effect of multiple doses of eight plant extracts (, , , , , , , ) on via LC-MS and the down-regulatory effect of them on , and genes involved in the aflatoxin B1 biosynthesis pathway using RT-qPCR analyses. Our results showed that (4 mg/mL), (6 mg/mL) and (2 mg/mL) completely stopped the production of aflatoxin B1, without inducing significant changes in growth.