Clinicians' perceptions for indicating and contra-indicating integrated treatment for SUD and comorbid PTSD, a vignette study.

Background: Posttraumatic Stress Disorder (PTSD) is more common in patients with Substance Use Disorder (SUD) than in the general population. Although international guidelines recommend integrated treatment clinicians are still hesitant in offering integrated treatment and more concrete recommendations are needed. This study aims to contribute to a practice-based guideline through the exploration of practice-based decision criteria to determine the indication and treatment of SUD and PTSD.

Treatment of adductor spasticity with BTX-A in children with CP: a randomized, double-blind, placebo-controlled study.

Adductor spasticity in children with cerebral palsy (CP) impairs motor function and development. In a placebo-controlled, double-blind, randomized multicentre study, we evaluated the effects of botulinum toxin A(BTX-A) in 61 children (37 males, 24 females; mean age 6 years 1 month [SD 3y 1mo]) with CP (leg-dominated tetraparesis, n=39; tetraparesis, n=22; GMFCS level I, n=3; II, n=6; III, n=17; IV, n=29; V, n=6). Four weeks after treatment, a significant superiority of BTX-A was observed in the primary outcome measure (knee-knee distance 'fast catch', p=0.

Interpersonal aspects of personality disorders in opioid-dependent patients: the convergence of the ICL-R and the SIDP-IV.

This study aims to establish the convergence of the empirically based Diagnostic Statistical Manual (DSM)- and theory-based interpersonal behavioral approaches to personality, in opioid-dependent patients (n = 110), with the use of the Structured Interview for DSM-IV Personality [Pfohl et al.: Structured Interview for DSM-IV PD. Iowa City, UICM, 1995] and the Interpersonal Checklist-Revised (ICL-R) [DeJong and VandenBrink: ICL-R.

[Use of computer applications to support clinical processes. An electronic letter of discharge as resource for DRG-relevant coding].

Not long ago forensic aspects where the major driving force to complete and correct medical documentation. Diagnosis related groups becoming basic data for billing of hospital treatment in Germany now extremely extend the need for complex administrative documentation over-ruling all medical documentary needs. Due to unchanged personnel resources in hospitals and due to a lack of comfortable tools supporting these documentary needs medical personnel in most hospitals is confronted with an annoying effort to fulfill these needs.

Trauma nursing in the German health care system.

This article provides an overview of the history and current practice of trauma nursing in the German health care system. A description of nursing education, skills, duties, and responsibilities of the nursing workforce is complemented by a brief description of the trauma system. As current demographic developments, structural changes, and medical progress result in a rapidly changing health care environment, tasks for nurses are becoming increasingly complex.

High throughput parallel analysis of hundreds of patient samples for more than 100 mutations in multiple disease genes.

As more mutations are identified in genes of known sequence, there is a crucial need in the areas of medical genetics and genome analysis for rapid, accurate and cost-effective methods of mutation detection. We have developed a multiplex allele-specific diagnostic assay (MASDA) for analysis of large numbers of samples (> 500) simultaneously for a large number of known mutations (> 100) in a single assay. MASDA utilizes oligonucleotide hybridization to interrogate DNA sequences.

A unique nucleoprotein structure associated with the Drosophila melanogaster 18-28 S rDNA nontranscribed spacer.

We have detected unique nucleoprotein particles specific for the 18-28 S rDNA nontranscribed spacer of Drosophila melanogaster. The particles migrate between di- and trinucleosomes on nucleoprotein gels, and are between mono- and dinucleosomal in DNA length. These migration properties suggest that the nontranscribed spacer particles could have a protein component larger than a histone core.

Induction of malignant transformation by various chemicals in Balb/3T3 clone A31-1-1 cells and biological characterization of some transformants.

Balb/c A31-1-1 cells were used for the study of transformation induction by chemicals with different mutagenic specificities. We show that survival of these cells and therefore the calculated transformation frequency per cells at risk is dependent upon the cell density at the time of treatment. It is suggested that equal cell densities should be used for measuring survival values and transformation induction.

Excretion of modified nucleosides during development of malignant lymphomas in mice after whole body irradiation.

During x-ray-induced development of malignant lymphomas in mice their urinary excretion of eight modified nucleosides was monitored and the values were compared to the results of the histological examination of the animals at time of their sacrifice. It was found that the pathologically augmented excretion of modified nucleosides begins as much as several weeks before the malignant lymphomas can be diagnosed clinically. Thus some mice had increased levels of modified nucleosides even 10 weeks before sacrifice, though at the time of sacrifice the histological investigation revealed only some small foci of reticulum cell neoplasm in their spleen.

A craniofacial growth maturity gradient for males and females between 4 and 16 years of age.

Differential growth of the craniofacial complex implies variation in ontogenetic patterns of development. This investigation quantifies the relative maturity--as defined by percent adult status--of nine cephalometric dimensions and stature. Analysis is based on 663 lateral cephalograms from a mixed longitudinal sample of 26 males and 25 females between 4 and 16 years of age.

Evaluation of a mutation test using S49 mouse lymphoma cells and monitoring simultaneously the induction of dexamethasone resistance, 6-thioguanine resistance and ouabain resistance.

A test for the detection of chemically induced mutants in S49 mouse lymphoma cells is described. These cells can be plated in parallel in several selective media; the induced frequencies of dexamethasone-resistant, 6-thioguanine-resistant and ouabain-resistant mutants were compared. The first two selection systems permit the detection of all kinds of mutation that result in alteration or partial or complete loss of the gene product concerned, whereas ouabain-resistant mutants can only be induced with strong point mutagens in these cells.

Increasing urinary levels of modified nucleosides and bases during tumor development in mice.

Based on the fact that human cancer patients excrete increased amounts of various modified nucleosides and bases in their urine, we investigated whether the same phenomenon takes place in mice bearing experimentally induced tumors. We did indeed find that mice with MCA-induced skin tumors and mice exhibiting leukemia after X-ray irradiation excrete severalfold higher levels of modified nucleosides and bases than do untreated control mice. Comparison of the time course of altered urinary excretion of these RNA catabolites with the appearance of a recognizable tumor after MCA application revealed that the onset of the altered excretion rate of these compounds precedes tumor diagnosis.

Craniofacial growth and size patterns during postnatal development.

Patterns of craniofacial growth and size relationships are described for a mixed longitudinal sample of 26 males and 25 females, followed serially from four years of age through adult status. The seven dimensions examined, derived from 663 lateral cephalograms, show differential patterns of negative allometric growth relative to statural increase. Relative growth is greatest for mandibular traits, followed by upper facial and neurocranial traits, respectively.

Increased urinary levels of RNA catabolites in mice as early indicators for malignant growth.

It is known that human cancer patients exhibit an altered urinary excretion pattern of modified nucleosides and bases, that mice bearing skin tumors excrete increased amounts of various modified nucleosides and bases, and that the onset of altered excretion of modified RNA constituents precedes tumor diagnosis. We have now obtained similar results in mice for lymphoblastic leukemia induced by a single exposure to X-ray irradiation. Mice showing severe leukemic symptoms excrete severalfold amounts of modified nucleosides in their 24-hr urine, when compared with untreated controls.

Principal components of craniofacial growth for white Philadelphia males and females between 6 and 22 years of age.

Three principal components, explaining 83 percent of the common variation for 999 males and females between 6 and 22 years of age, describe ontogenetic patterns of relationship for seven facial dimensions, including sella-nasion, sella-basion, nasion-prosthion, infradentale-menton, articulare-gnathion, gonion-gnathion, and articulare-gonion. Accounting for 65 percent of the variation, a general component associated with both size and shape defines size-required changes in proprotion during growth. Independent patterns of regional variation associated with alveolar remodeling (second component) and condylar growth (third component) describe specific sources of facial modification.

Elevated urinary excretion of RNA catabolites as an early signal of tumor development in mice.

Determination of the urinary excretion rates of 12 modified nucleosides and bases in mice after tumor induction by application of a single dose of 3-methylcholanthrene revealed that mice bearing tumors in advanced stages excrete many-fold amounts of these nucleic acid catabolites compared with the control values. The excretion rate of several of these nucleic acid constituents like ac4C, m1A, PsU, and m2Gua increased before the tumor was diagnosable. Untreated control mice as well as mice having received the carcinogen, but not developing a tumor, did not show an alteration in the excretion values of any of the modified nucleosides and bases determined.

Genetic analysis of mutations causing borrelidin resistance by overproduction of threonyl-transfer ribonucleic acid synthetase.

Mutations leading to borrelidin resistance in Escherichia coli by overproduction of threonyl-transfer ribonucleic acid synthetase were anaylzed genetically. The regulatory mutations were closely linked to the treonyl-transfer ribonucleic acid synthetase structural gene (thrS), located clockwise to it. The mutation that causes the threefold-increased enzyme level was more distant from thrS than the mutation responsible for the ninefold overproduction.

Non-coordinate regulation of enzymes involved in transfer RNA metabolism in Escherichia coli.

During different steady state growth conditions in Escherichia coli the level of the three tRNA-modifying enzymes, the tRNA(m5Urd)-, tRNA(m1Guo)- and tRNA(mam5s2Urd)methyltransferase and of five aminoacyl-tRNA synthetases, the leucyl-, valyl-, isoleucyl-, arginyl- and threonyl-tRNA-synthetase, has been determined. It is shown that those two classes of tRNA affecting enzymes are not coordinately regulated and that even within these two groups of enzymes the constituents are regulated independently of each other. Furthermore it is demonstrated that none of the aminoacyl-tRNA synthetases and only one of the three tRNA-methyltransferases, the tRNA(m5Urd)methyltransferase, is under control of the relA+-gene.

Escherichia coli mutants overproducing phenylalanyl- and threonyl-tRNA synthetase.

The structural genes for threonyl-tRNA synthetase (ThrRS) and phenylalanyl-tRNA synthetase (PheRS) are closely linked on the Escherichia coli chromosome. To study whether these enzymes share a common regulatory element, we have investigated their synthesis in mutants which were selected for overproduction of either ThrRS or PheRS. It was found that mutants isolated previously for overproduction of ThrRS as strains resistant to the antibiotic borrelidin (strains Bor Res 3 and Bor Res 15) did not show an elevated level of PheRS.

Alteration of the intracellular concentration of aminoacyl-tRNA synthetases and isoaccepting tRNAs during amino-acid limited growth in Escherichia coli.

Growth of Escherichia coli AB 2271 under threonine or isoleucine deficiency leads to a depression of the threonyl-tRNA synthetase and isoleucyl-tRNA synthetase respectively. During this amino-acid-limited growth the concentrations of isoaccepting fractions of the cognate tRNA species were changed, as demonstrated by their altered reversed-phase-5 chromatograms. But, in addition, the profiles of the isoacceptors of all other tRNA species investigated, i.

Genetically determined differences in concentrations of isoaccepting tRNAs in Escherichia coli.

Two examples of genetically determined altered concentrations of isoaccepting tRNAs are presented. The concentrations of isoaccepting tRNAsThr are selectively changed by a mutation causing a fourfold overproduction of the cognate aminoacyl-tRNA-synthetase, the threonyl-tRNA synthetase, whereas the distribution of isoaccepting tRNAs of four control tRNA-species in these E. coli mutants was not affected by that mutation.

Threonyl-transfer ribonucleic acid synthetase from Escherichia coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing lambda bacteriophage.

Threonyl-transfer ribonucleic acid synthetase (ThrRS) has been purified from a strain of Escherichia coli that shows a ninefold overproduction of this enzyme. Determination of the molecular weight of the purified, native enzyme by gel chromatography and by polyacrylamide gel electrophoresis at different gel concentrations yielded apparent molecular weight values of 150,000 and 161,000, respectively. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate yields a single protein band of 76,000-dalton size.

Intra-group variations in the dental eruption sequence of Macaca fuscata fuscata.

The purpose of this investigation was to establish general trends of intra-group variations in the dental eruption sequence of a semi-wild troop of Maca fuscata fuscata. Dental impressions were taken on 128 animals of varying ages. The analysis of the dental casts revealed sexual dimorphism in sequence and timing of eruption.