Flavopiridol restores granulopoiesis in experimental models of severe congenital neutropenia.

Severe congenital neutropenia (CN) patients require life-long treatment with recombinant human granulocyte colony-stimulating factor (rhG-CSF), but some show no response. We sought to establish a therapy for CN that targets signaling pathways causing maturation arrest of granulocytic progenitors. We developed an isogenic induced pluripotent stem cell (iPSC) in vitro model of CN associated with ELANE mutations (ELANE-CN) and performed an in silico drug repurposing analysis of the transcriptomics of iPSC-generated hematopoietic stem and progenitor cells.

Sericin coats of silk fibres, a degumming waste or future material?

Silk is a fibrous biopolymer with a recorded history in the textile industries for centuries. This fibre is constituted of two different proteins: fibroin and sericin, of which the latter accounting for approximately 20-30 % of the silk mass. Silk sericin (SSER) was previously considered as a waste by-product in silk fibroin extraction.

Food applications of bioactive biomaterials based on gelatin and chitosan.

Food packaging must guarantee the products' quality during the different operations including packing and maintenance throughout transportation and storage until to consumption. Thus, it should satisfy, both, food freshness and quality preservation and consumers health safety. Natural bio-sourced polymers have been explored as safe edible materials for several packaging applications, being interestingly carrier of bioactive substances, once added to improve films' properties.

Social connectedness and loneliness in school for autistic and allistic children.

Many previous studies reported that autistic children have fewer social connections. Yet, recent studies also show that autistic children more often feel lonely in school than allistic (i.e.

naRNA-LL37 composite DAMPs define sterile NETs as self-propagating drivers of inflammation.

Neutrophil extracellular traps (NETs) are a key antimicrobial feature of cellular innate immunity mediated by polymorphonuclear neutrophils (PMNs). NETs counteract microbes but are also linked to inflammation in atherosclerosis, arthritis, or psoriasis by unknown mechanisms. Here, we report that NET-associated RNA (naRNA) stimulates further NET formation in naive PMNs via a unique TLR8-NLRP3 inflammasome-dependent pathway.

Bacillus methylotrophicus DCS1: Production of Different Lipopeptide Families, In Vitro Antifungal Activity and Suppression of Fusarium Wilt in Tomato Plants.

The present work aims to quantitatively and qualitatively monitor the production of lipopeptide mixtures by Bacillus methylotrophicus DCS1 strain in Landy medium and to investigate the antifungal activities of DCS1 strain and its produced lipopeptides. The in vitro activities were tested by the direct confrontation and agar well diffusion methods, while the in vivo study was carried out in order to test the efficiency of DCS1 bacterial suspension in the control of Fusarium wilt in tomato plants. Identification of lipopeptides by mass spectrometry (LC/MSD-TOF) showed that lipopeptide isoforms produced during the first 24 h and 48 h of fermentation are identical, belonging to bacillomycin D and fengycins A and B homologues with a difference in the yield of production.

CRISPR-Cas9n-mediated ELANE promoter editing for gene therapy of severe congenital neutropenia.

Severe congenital neutropenia (CN) is an inherited pre-leukemia bone marrow failure syndrome commonly caused by autosomal-dominant ELANE mutations (ELANE-CN). ELANE-CN patients are treated with daily injections of recombinant human granulocyte colony-stimulating factor (rhG-CSF). However, some patients do not respond to rhG-CSF, and approximately 15% of ELANE-CN patients develop myelodysplasia or acute myeloid leukemia.

Differential transcriptional control of hematopoiesis in congenital and cyclic neutropenia patients harboring mutations.

Mutations in the ELANE gene, encoding the neutrophil elastase (NE) protein, are responsible for most cyclic neutropenia (CyN) cases and approximately 25% of congenital neutropenia (CN) cases. In CN and in CyN, a median of 2.8% of CD34+ cells were early CD49f+ hematopoietic stem cells (eHSC) that did not express ELANE and thus escape from the unfolded protein response (UPR) caused by mutated NE.

Social connectedness at the playground before and after COVID-19 school closure.

Social connectedness at school is crucial to children's development, yet very little is known about the way it has been affected by school closures during COVID-19 pandemic. We compared pre-post lockdown levels of social connectedness at a school playground in forty-three primary school-aged children, using wearable sensors, observations, peer nominations and self-reports. Upon school reopening, findings from sensors and peer nominations indicated increases in children's interaction time, network diversity and network centrality.

The impact of loose-parts-play on schoolyard social participation of children with and without disabilities: A case study.

Background: Outdoor social participation in the school playground is crucial for children's socio-emotional and cognitive development. Yet, many children with disabilities in mainstream educational settings are not socially included within their peer group. We examined whether loose-parts-play (LPP), a common and cost-effective intervention that changes the playground play environment to enhance child-led free play, can promote social participation for children with and without disabilities.

Alleviating the adverse effects of salinity stress on Salicornia persica using sodium nitroprusside and potassium nitrate.

Background: Glasswort (Salicornia persica) is identified as a halophyte plant, which is one of the most tolerant plants to salt conditions. The seed oil of the plant contains about 33% oil. In the present study, the effects of sodium nitroprusside (SNP; 0, 0.

Correction: Nasri et al. A Novel Data-Driven Approach to Examine Children's Movements and Social Behaviour in Schoolyard Environments. 2022, , 1177.

The authors request the following corrections because the changes made according to the second round of the review process were not included in the original publication [...

Effect of Potassium Deficiency on Physiological Responses and Anatomical Structure of Basil, L.

The aim of this study was to investigate the effect of a variable supply of potassium to culture medium on physiological and anatomical parameters (histological sections at the third internode) in basil, . Thirty-four-day-old plants grown on basic nutrient medium were divided into four batches and grown on media with varying doses of potassium: 0.375 mM, 0.

A Novel Data-Driven Approach to Examine Children's Movements and Social Behaviour in Schoolyard Environments.

(1) Many children in schoolyards are excluded from social interactions with peers on a daily basis. For these excluded children, schoolyard environments often contain features that hinder, rather than facilitate, their participation. These features may include lack of appropriate play equipment, overcrowded areas, or insufficient supervision.

Regenerative medicine meets translational oncology: Modeling leukemic bone marrow niche.

Most studies on leukemia focus on leukemia cells as isolated objects without considering the bone marrow niche. Pal et al. have recreated the bone marrow niche using induced pluripotent stem cells (iPSCs), identifying CDH2 as a therapeutically druggable leukemia-promoting factor.

The effect of ascorbic acid and bio fertilizers on basil under drought stress.

Evaluate the effect of ascorbic acid application and coexistence of Mycorrhiza fungus and Azospirillium on basil (Ocimum basilicum L.) under drought stress. This experiment was performed as a split factorial in a randomized complete block design with three replications in the crop year 2017-2018 in Shahriar, Iran.

Using ordered and unordered logistic regressions to investigate risk factors associated with pedestrian crash injury severity in Victoria, Australia.

Introduction: The safety of pedestrians is a major concern in Victoria, Australia. Despite the considerable number of pedestrian fatalities and injuries in traffic crashes, a limited number of studies focused on pedestrian crash severity in Victoria.

Methods: This study investigates and identifies the influential factors determining the severity of pedestrian injuries in traffic crashes in Victoria by using crash data from 2010 to 2019.

Exogenous glutathione protects against gentamicin-induced acute kidney injury by inhibiting NF-κB pathway, oxidative stress, and apoptosis and regulating PCNA.

This study was designed, for the first time, to examine the possible nephroprotective effects of exogenous glutathione (EGSH) (100 mg/kg, intraperitoneally) on gentamicin-induced acute kidney injury (GM-induced AKI). EGSH reduced renal histopathological changes, inflammatory cell infiltration, and improved renal dysfunction in rats with AKI. EGSH ameliorated GM-induced renal oxidative stress by promoting the renal activities of catalase, glutathione peroxidase, and superoxide dismutase and diminishing renal malondialdehyde and serum nitric oxide levels.

Impact of Magnesium Salt on the Mechanical and Thermal Properties of Poly(vinyl alcohol).

The effects of magnesium salts with various anion species on the structure and properties of a poly(vinyl alcohol) (PVA) film were studied. The glass transition temperature of the PVA film increased following the addition of a magnesium salt. Furthermore, the salt greatly enhanced the modulus and yield stress and reduced the crystallinity of the film.

A Thyroid Stimulating Hormone Reference Range: Iranian Thyroid Cohort study.

Background: Current reference values for thyroid function tests are derived from data from different ethnicities and geographical areas. In this article, we aim to select criteria from the guidelines proposed by the National Academy of Clinical Biochemistry (NACB) and to determine the TSH and T4 reference limits in the iodine-sufficient area of Isfahan, a metropolitan city in Iran.

Materials And Methods: This study was conducted within the framework of "Isfahan Thyroid Study (ITS)", an ongoing prospective cohort that started in 2006 (n=2523) until 2011 (n=711) and included participants above the age of twenty.

Development and characterization of fish gelatin-based biodegradable film enriched with extract as active packaging for cheese preservation.

The physical and functional properties of gelatin-based films enriched with organic extracts from seeds were studied. Gelatin was extracted from the skin of dogfish () and the functional gelatin-based films were used to preserve cheese during chilled storage. Ethanol extract (LSE3) and gelatin-based film enriched with LSE3 at 20 μg/mL showed high antioxidant potential using various complementary methods.