Healing of intrabony defects and its relationship to root canal therapy. A histologic and histometric study in dogs.

The purpose of this study was to evaluate the importance of root canal therapy in the healing process of severe intrabony defects. Four beagle dogs were used and 32 interproximal intrabony defects, up to the apical third, were created. Wire ligatures were placed into these defects for plaque accumulation.

Vascular response to guided tissue regeneration procedures using nonresorbable and bioabsorbable membranes in dogs.

Revascularization of the periodontal tissues was studied following guided tissue regeneration (GTR) procedures using both nonresorbable and bioabsorbable membranes. The procedures were performed in 8 female beagle dogs, 4 to 6 years old. Second, third, and fourth mandibular premolars were involved; experimental periods covered from 3 days to 49 days.

Vascular changes following mucoperiosteal flap surgery: a fluorescein angiography study in dogs.

This study was undertaken to determine the vascular changes which occur following mucoperiosteal flap surgery where two different suturing techniques were employed. In four healthy adult mongrel dogs, buccal and lingual full-thickness envelope flaps were reflected in the mandibular quadrants following intravicular incisions from the first premolar to the first molar. The flaps were immediately readapted and primary closure was achieved by the horizontal mattress suturing technique in one quadrant and the direct interrupted suturing technique in the contralateral quadrant of each dog.

Guided tissue regeneration: comparison of bioabsorbable and non-bioabsorbable membranes. Histologic and histometric study in dogs.

This study examined histologic and histometric responses to 2 bioabsorbable membranes made from a synthetic copolymer of glycolide and lactide. They were tested for their biocompatibility, resorption characteristics, and ability to support periodontal regeneration. Expanded polytetrafluoroethylene (ePTFE) was used as control.

Guided Tissue Regeneration and Bone Grafts in the Treatment of Furcation Defects.

The present study evaluated the effects of guided tissue regeneration (GTR), with and without demineralized freeze-dried cortical bone grafts, in the treatment of furcation defects in 4 female beagle dogs with naturally occurring periodontal disease. The root surfaces were thoroughly debrided. Four weeks later, full thickness facial and lingual mucoperiosteal flaps were reflected using inverse bevel incisions on both sides of the mandible involving the 2nd, 3rd, and 4th premolar, and the 1st molar teeth.

Guided tissue regeneration and bone grafts in the treatment of furcation defects.

The present study evaluated the effects of guided tissue regeneration (GTR), with and without demineralized freeze-dried cortical bone grafts, in the treatment of furcation defects in 4 female beagle dogs with naturally occurring periodontal disease. The root surfaces were thoroughly debrided. Four weeks later, full thickness facial and lingual mucoperiosteal flaps were reflected using inverse bevel incisions on both sides of the mandible involving the 2nd, 3rd, and 4th premolar, and the 1st molar teeth.

Guided tissue regeneration with dentin biomodification.

This study was undertaken to provide histologic observations on the effectiveness of citric acid and tetracycline HCl root surface demineralization when used in conjunction with guided tissue regeneration (GTR) procedures. Eight beagle dogs with naturally-occurring periodontitis were initially treated with scaling and root planing. Four weeks later, 12 mandibular quadrants were treated surgically with modified Widman flap and expanded polytetrafluoroethylene (ePTFE).

Histologic and histometric responses to polymeric composite grafts.

The present study was designed to determine whether a polymeric composite promotes new attachment in artificially-induced bony defects in the dog model. HTR, hard tissue replacement, is a non-resorbable calcium-layered polymer of polymethyl-methacrylate and hydroxyethyl-methacrylate. It has been reported to be clinically non-inflammatory, osteophilic, and osteoconductive.

In vitro effects of citric acid application techniques on dentin surfaces.

The present study evaluated the in vitro effects of different application techniques of citric acid on dentin root surfaces. Ten freshly extracted, periodontally involved teeth were obtained and 4 dentin slabs, approximately 4 x 6 x 2 mm, were obtained from the roots of each tooth, for a total of 40 slabs. These slabs were identified by tooth and preserved in 1:1 anhydrous glycerol/absolute alcohol solution.

Correlation of periodontal probe penetration and degree of inflammation.

This histological study correlated the depth of probe penetration to periodontal health in mongrel dogs. Various probing forces were also applied with an electronic pressure-sensitive probe and compared to manual probing. Three adult mongrel dogs with naturally occurring periodontitis in at least one quadrant were selected and gross scaling was only performed in these diseased areas.

Periodontal healing following guided tissue regeneration with citric acid and fibronectin application.

This study was undertaken to determine the effects of guided tissue regeneration (GTR) with and without citric acid conditioning and autologous fibronectin application. The study subjects were four female beagle dogs with spontaneous periodontitis. The dogs were given thorough root debridement and 4 weeks later, mucoperiosteal flaps were raised on both sides of the mandible involving the 2nd, 3rd, and 4th premolar and 1st molar teeth.

Furcation defects in dogs treated by guided tissue regeneration (GTR).

This study evaluated the effects of guided tissue regeneration (GTR) in the treatment of Class II furcation defects in beagle dogs. Gore-Tex periodontal material was used, and the amount of furcation fill and the surface area corresponding to new connective tissue attachment and new bone were evaluated. If present, epithelium was also evaluated.

New attachment achieved by guided tissue regeneration in beagle dogs.

This study tested the effects of using a Gore-Tex periodontal material on new connective tissue attachment in beagle dogs with natural periodontitis. Seven female beagle dogs with advanced periodontitis were selected and given thorough root debridement. Under general anesthesia four weeks later, mucoperiosteal flaps were raised involving all mandibular premolars and first molars.

Effect of citric acid and lyophilized autologous plasma on healing following periodontal flap surgery in monkeys.

The purpose of this histologic, histometric, and autoradiographic study was to examine the effect of citric acid conditioning and lyophilized autologous plasma (LAP) application on healing following periodontal flap surgery. Mucoperiosteal flaps were elevated in six rhesus monkeys using the modified Widman flap procedure. A total of 24 quadrants were treated, each included the first and second premolar and first and second molar teeth.

Effect of citric acid and various concentrations of fibronectin on healing following periodontal flap surgery in dogs.

The purpose of this histologic and histometric study was to examine the effect of citric acid and increasing concentrations of fibronectin on new connective tissue attachment following periodontal flap surgery. Full thickness, mucoperiosteal flaps were elevated in six healthy mongrel dogs. Two to 3 mm of alveolar bone were removed along the buccal aspect of the teeth in the mandible and into the interproximal areas of each surgical site.

Cell proliferation after flap surgery, root conditioning and fibronectin application.

This study evaluated the effects of citric acid demineralization and autologous fibronectin application on cell proliferation after mucoperiosteal flap surgery. Three adult rhesus monkeys were used. After flaps were raised, the roots were surgically exposed and planed.

Effects of citric acid and fibronectin and laminin application in treating periodontitis.

To determine the effect on new connective tissue attachment of citric acid conditioning and fibronectin-laminin application in treating naturally occurring periodontitis, all 4 quadrants in each of 2 Beagle dogs were used. Each quadrant included: P2, P3, P4, and M1 teeth. 2 treatment modalities were employed and comparatively analyzed for differences in histological healing respponses at 120 days after surgery.

Effect of fibronectin on healing of replanted teeth in monkeys: a histologic and autoradiographic study.

The purpose of this study was to evaluate the effect of fibronectin application on healing of replanted teeth. Three rhesus monkeys were used. Maxillary and mandibular incisors and premolars were extracted and replanted.

Effect of lyophilized autologous plasma on periodontal healing of replanted teeth.

The purpose of this histologic and autoradiographic study of replanted teeth was to evaluate the beneficial effect, if any, of lyophilized autologous plasma (LAP) application on periodontal healing and to re-examine rates of repair in different areas of the associated periodontium following replantation. Maxillary and mandibular incisors and premolars of three rhesus monkeys were used. Teeth were extracted with forceps and placed in sterile physiologic saline.

Healing and revascularization of apical periodontium and dental pulps in apicoectomized and nonapicoectomized tooth replants in monkeys. A study of fully developed teeth.

This report primarily concerns an experimental study of the effect of apicoectomy on the healing and revascularization of the periodontal membrane and dental pulps in tooth replants. Five rhesus monkeys (Macaca mulatta) were subjected to surgical procedures in which twenty incisor teeth were extracted and immediately replanted. Ten of these incisors were subjected to apicoectomy.

The effect of citric acid and fibronectin application on healing following surgical treatment of naturally occurring periodontal disease in beagle dogs.

It has recently been suggested that following the exposure of root surface collagen with citric acid, the addition of topically applied fibronectin might promote healing with a fibrous re-attachment. The purpose of this study was to determine the benefit of citric acid demineralization and fibronectin application in the surgical treatment of severe, naturally occurring periodontal disease in Beagle dogs. The 4 treatment modalities employed were: (1) surgery alone (mucoperiosteal flaps); (2) surgery plus fibronectin; (3) surgery plus citric acid; (4) surgery plus citric acid followed by fibronectin application.

Histopathologic findings in temporomandibular joints of aged individuals.

Twenty-four TMJs from well preserved 62- to 97-year-old human cadavers, 12 men and 12 women, were used in this study. The TMJs were routinely processed to identify (1) the histologic and histopathologic changes present in the articulations and (2) the components of the joint most frequently affected by these changes. The most significant histopathologic findings were (1) intense fibrocartilaginous proliferations of the condylar head and articular eminence linings, (2) neovascularization or myxomatoid degenerative changes in articular disks, and (3) proliferation of synovial villi, subsynovial tissue, and muscular fibers.

Carrageenan-induced inflammation and its effects on mitotic activity and keratinization of gingival epithelium. A histologic and autoradiographic study.

This study was undertaken in order to: evaluate the cell population in carrageenan-induced inflammation and investigate the extent to which this inflammation modified mitotic activity, and keratinization of the sulcular epithelium induced by daily prophylaxes in monkeys. Normal-keratinized oral gingival epithelium was also evaluated for these processes in the same gingival specimens. Each of three adult Rhesus monkeys received a thorough prophylaxis 1 week prior to the experiment.

Dextran-induced inflammation and its effect on keratinized gingival epithelium in monkeys.

The cell population present during dextran-induced inflammation and its effect upon induced keratinization of the sulcular epithelium was investigated in two young adult male Rhesus monkeys. Keratinization of the sulcus epithelium was induced by a combined regimen of scaling, an intravenous injection of achromycin and daily rubber cup prophylaxes. After keratinization was confirmed by means of biopsies, inflammation was induced either by injecting 200 microliters of a 5% dextran saline solution or by applying the solution topically on the marginal gingiva for 2 weeks.