Serological survey of Toxoplasma gondii infection in a breeding kennel with reproductive disorders.

Introduction: Toxoplasma gondii (T. gondii) infection in dogs is distributed globally. Given the potential impact of T.

Brain -cyst-driven genes expression in Toxoplasma Gondii Tehran strain: a parasitic-immunogenicity assessment by dint of RNA-Seq.

Toxoplasma gondii (T. gondii) is an obligate intracellular parasite of warm-blooded vertebrates. At present, High-throughput RNA sequencing analysis have made it possible to determine the role of effective genes in host immune response.

Molecular, Morphological, and Spatial Study of (Pulmonata, Lymnaeidae) from Southeastern Iran.

Background: Snails of the genus are the intermediate hosts of species the etiological agents of liver fluke disease, fascioliasis. A genetically different but morphologically very similar species in the genus, is sympatrically distributed with in some regions of the world. We aimed to investigate the occurrence of in Kerman province, Iran and to characterize genetically specimens from southeast Iran.

Prophylactic Activity of Biogenic Selenium Nanoparticles Against Chronic Infection.

Background: Studies showed that biogenic selenium nanoparticles (SeNPs) have a number of pharmacological properties, such as antimicrobial ones.

Objective: The present investigation assesses the efficacy of biogenic selenium nanoparticles (SeNPs) as a new patent against latent toxoplasmosis in a mice model.

Methods: Male BALB/c mice were orally treated with SeNPs at the doses of 2.

Biogenic selenium nanoparticles target chronic toxoplasmosis with minimal cytotoxicity in a mouse model.

Nanoparticles (NPs) have numerous biological benefits due to their large surface-volume ratio and convenient entry into cells compared to other particles. Previous research has shown the antimicrobial properties of biogenic selenium NPs (SeNps) and their effects on cellular immunomodulatory cytokines that play a key role in controlling infections. This study aimed to evaluate the therapeutic effects of SeNPs against chronic toxoplasmosis in mice.

The First Survey of Isolation and Molecular Typing of by Bioassay and PCR Method in BALB/c Mice in Camels () from Eastern Iran.

Background: We isolated from camels by bioassay method in mice model and detect parasitic DNA in brain mice by molecular methods.

Methods: One hundred tissue samples including heart (n=50), and diaphragm (n=50) were collected from camels (n=50) slaughtered in abattoirs from Feb to Oct 2015 in three provinces located in eastern Iran. In first, blood sample from 50 camels was assayed for anti- antibodies by modified agglutination test (MAT) test.

Propranolol efficacy as a novel adjuvant for immunization against Toxoplasma gondii tachyzoites.

Severe or lethal damages, caused by Toxoplasma gondii infection in congenital cases and immunocompromised patients implies the necessity for development of a vaccine and an appropriate adjuvant would be needed to elicit a protective Th1 biased-immune response. The adjuvant activity of propranolol was surveyed and compared with alum by immunization of BALB/c mice with protein components of T. gondii tachyzoites.

Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran.

Objective: The purpose of the present study was morphometric and molecular characterization of Fasciola isolates from ruminants in Iran.

Methods: Flukes were collected from the livers of 54 naturally infected sheep and cattle. The proportion of body length to width (L/W) of each fresh fluke was measured using a digital caliper.

Prevalence of Infection among Healthy Blood Donors in Northeast of Iran.

Background: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti- antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015.

Methods: Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti- antibodies by enzyme-linked immunosorbent assay (ELISA). A structured questionnaire was used to obtain information on risk factors for infection.

Considerable Genetic Diversity of Clinical Isolates in a Targeted Population in South of Iran.

Background: The present study aimed to characterize genetically and to compare the most frequently occurring strains of isolated from southern Iran.

Methods: Totally, 150 vaginal swab and urine specimens were collected from symptomatic and asymptomatic women from May 2012 to Jun 2013. This study implemented a sensitive and reliable PCR-restriction fragment length polymorphism (RFLP) typing method on the actin gene.

Acetylcholinesterase Inhibitor Improves Learning and Memory Impairment Induced by Infection.

Background: Here, we established the mouse models of chronic toxoplasmosis by Tehran strain to provide a good understanding about defining the possible association between exposure and learning and memory impairments. Moreover, as secondary objective of the present study, we hypothesized whether administration of an acetylcholinesterase (AChE) inhibitor could reduce learning and memory impairments induced by infection.

Methods: Twenty-four male BALB/c mice were used to establishment of latent toxoplasmosis.

Seroprevalence of infection among childbearing age women in Kerman city, southeastern Iran.

This cross-sectional study aims to determine the prevalence of IgM and IgG anti-. antibodies and the associated risk factors among childbearing age women referring to counseling centers before marriage in Kerman city, southeast of Iran. Totally, 300 serum samples were collected from women referred to Central Laboratory for Marriage Consultation in Kerman city were screened for IgG and IgM anti- antibodies by enzyme linked immunosorbent assay (ELISA).

Adaptive immune response in symptomatic and asymptomatic enteric protozoal infection: evidence for a determining role of parasite genetic heterogeneity in host immunity to human giardiasis.

The genetic basis of the ultimate clinical outcomes of human giardiasis has been the subject of numerous investigations. We previously demonstrated roles for both host and parasite factors in determining the outcome of enteric infection in a murine model of Giardia duodenalis infection. In the current study, fecal and serum specimens from healthy controls and human subjects infected with the intestinal parasite G.

Toxoplasma gondii Infection Potentiates Cognitive Impairments of Alzheimer's Disease in the BALB/c Mice.

This study tests the hypothesis that in chronic Toxoplasma gondii infection communication among immune cells promotes neuroinflammation through cytokine networks and potentiate cognitive impairments in BALB/c mice with Alzheimer's disease (AD). The animal model of Toxoplasma infection was established by the intraperitoneal inoculation of 20-25 tissue cysts from the Tehran strain of T. gondii .

The possible association between Toxoplasma gondii infection and risk of anxiety and cognitive disorders in BALB/c mice.

There are conflicting reports concerning the association of Toxoplasma gondii infection with increased risk of mental disorders. This investigation will provide a good understanding about defining the possible association between T. gondii exposure and risk of anxiety and cognitive alterations.

Efficacy of the Bunium persicum (Boiss) Essential Oil against Acute Toxoplasmosis in Mice Model.

Background: We evaluated the in vivo activity of Bunium persicum (Boiss) essential oil on infected mice with acute toxoplasmosis.

Methods: To evaluate prophylactic effects, male NMRI mice received B. persicum essential oil at the concentrations of 0.

Toxoplasma gondii Infection Promotes Neuroinflammation Through Cytokine Networks and Induced Hyperalgesia in BALB/c Mice.

We hypothesized that in Toxoplasma gondii infection, communication among immune cells promotes neuroinflammation through cytokine networks and induces pain sensitivity under conditions of neuropathic pain. The animal model of Toxoplasma infection was established by the intraperitoneal inoculation of 20-25 tissue cysts from Tehran strain of T. gondii to BALB/c mice.

Molecular and serological detection of acute and latent toxoplasmosis using real-time PCR and ELISA techniques in blood donors of rafsanjan city, iran, 2013.

Background: The differentiation between acute and latent forms of the Toxoplasma gondii (T. gondii) infection is still considered as a complicated issue. This study was aimed to elucidate the status of infection in the blood donors and the probable importance of blood transfusion in the transmission of the infection through detecting both immunological and genetic markers of acute and latent infection.

In Vitro Inhibitory Effect of Berberis vulgaris (Berberidaceae) and Its Main Component, Berberine against Different Leishmania Species.

Background: Leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. The present study was aimed to investigate antileishmanial effects of various extracts of Berberis vulgaris also its active compoenent, berberine against Leishmania tropica and L. infantum species on in vitro experiments.

Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts.

Cystic echinococcosis (hydatid cyst, CE) as a zoonotic parasitic infection caused by the larval stage of the dog tapeworm Echinococcus granulosus is still an important economic and public health concern in the world. One of the treatment options for CE is surgical removal of the cysts combined with chemotherapy using albendazole and/or mebendazole before and after surgery. Currently, many scolicidal agents, which have some complications, have been used for inactivation of the cyst contents.

High resolution melting technique for molecular epidemiological studies of cystic echinococcosis: differentiating G1, G3, and G6 genotypes of Echinococcus granulosus sensu lato.

Reliable and rapid genotyping of large number of Echinococcus granulosus sensu lato isolates is crucial for understanding the epidemiology and transmission of cystic echinococcosis. We have developed a method for distinguishing and discriminating common genotypes of E. granulosus s.

Prevalence of anti-Toxoplasma gondii antibodies in sport horses from Qazvin, Iran.

In the present study, the prevalence of antibodies to Toxoplasma gondii in sport horses of Qazvin was examined using modified agglutination test (MAT). On 52 horse sera totally examined for anti-Toxoplasma antibodies, 37 horses (71.2%) were seropositive by MAT.

Feline immunodeficiency virus, feline leukemia virus and Toxoplasma gondii in stray and household cats in Kerman-Iran: seroprevalence and correlation with clinical and laboratory findings.

This study was carried out to determine the seroprevalence of feline leukemia virus (FeLV), feline immunodeficiency virus (FIV) and Toxoplasma gondii (T. gondii) infection among stray and owned cats in southeastern Iran and to identify the influence of age, sex, lifestyle, health status, and laboratory findings on seropositivity. The overall infection rate for FIV, FeLV, and T.