Facilitating high-force single-polysaccharide stretching using covalent attachment of one end of the chain.

Single polysaccharide force spectroscopy has yielded particularly interesting data, the interpretation of which requires the marriage of statistical-mechanical theories of polymer physics to the complexities afforded by possible force-induced conformational transitions of the constituent sugar rings. However, the difficulty of designing handles for the specific attachment of the different ends of polysaccharide chains to substrates, such as piezoelectric scanners, cantilevers or microbeads has meant that the majority of studies to date have been carried out with the polymer physisorbed to the substrates between which it is stretched, or at best chemically attached via bonds formed at uncontrolled locations along the length of the molecule. This means that the lengths of obtained polysaccharide stretches, as well as the forces that can be placed on the molecule without generating detachment, are generally smaller than those obtainable for polymers that offer the ability to be covalently attached to substrates specifically at their ends.