Construction of a species-specific vector for improved plastid transformation efficiency in L.

In the present study, we focused on designing a species-specific chloroplast vector for L. and finding out its transformation efficiency compared to a heterologous vector. The plastid transformation vector (CaIA) was designed to target homologous regions and of IR region.

Construction of chloroplast transformation vector and its functional evaluation in L.

Chloroplast transformation vectors require an expression cassette flanked by homologous plastid sequences to drive plastome recombination. The 16-23 plastome region was selected and using this region, a new species-specific plastid transformation vector CuIA was developed with pKSII as a backbone by inserting the 16- and -23 sequences from L. An independent expression cassette with gene encoding aminoglycoside 3'-adenylyltransferase with controlling elements is added into the - intergenic region that confers resistance to spectinomycin.

Efficient genetic transformation of L. by microprojectile bombardment.

Here, we report the optimized conditions for biolistic particle delivery-mediated genetic transformation of bitter melon using petiole segments. In this study, DNA-coated gold particles of 0.6 µm were used for optimizing the parameters of transformation and eventually regeneration of bitter melon putative transgenics.

Stable plastid transformation in L.

In the present investigation we report stable plastid transformation in L., a versatile medicinal herb via particle gun method. The vector KNTc, harbouring as a selectable marker and as a reporter gene which were under the control of synthetic promoter pNG1014a, targets inverted repeats, / of the plastid genome.

Biolistic transformation of Scoparia dulcis L.

Here, we report for the first time, the optimized conditions for microprojectile bombardment-mediated genetic transformation in Vassourinha (Scoparia dulcis L.), a Plantaginaceae medicinal plant species. Transformation was achieved by bombardment of axenic leaf segments with Binary vector pBI121 harbouring β-glucuronidase gene (GUS) as a reporter and neomycin phosphotransferase II gene (npt II) as a selectable marker.