Evaluation of Antitumor Activity of Hesperetin-Loaded Nanoparticles Against DMBA-Induced Oral Carcinogenesis Based on Tissue Autofluorescence Spectroscopy and Multivariate Analysis.

The present study is designed to understand the nature of endogenous fluorophores and cellular metabolism that occur in the experimental oral carcinogenesis and to assess their feasibility for antitumor efficacy of hesperetin-loaded nanoparticles (HETNPs) in comparison with native hesperetin (HET) against 7,12-dimethyl benz(a) anthracene (DMBA)-induced oral carcinogenesis using fluorescence spectroscopy. The fluorescence emission spectra of the control and the experimental buccal mucosa are recorded at an excitation wavelength of 320 nm with an emission ranging from 350 to 550 nm. The results show that there is a reduced contribution from the emission of collagen, nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), in DMBA-induced tumor tissues as compared with the control tissues.

Chemopreventive efficacy of naringenin-loaded nanoparticles in 7,12-dimethylbenz(a)anthracene induced experimental oral carcinogenesis.

Nanochemoprevention has been introduced recently as a novel approach for improving phytochemicals bioavailability and anti-tumor effect. The present study is designed to evaluate the chemopreventive efficacy of prepared naringenin-loaded nanoparticles (NARNPs) relative to efficacy of free naringenin (NAR) against 7,12-dimethyl benz(a)anthracene (DMBA)-induced oral carcinogenesis by evaluating the status of lipid peroxidation, antioxidants and immunoexpression patterns of proliferating cell nuclear antigen (PCNA) and p53 proteins. Transmission electron microscope (TEM) and dynamic light scattering (DLS) investigations have confirmed a narrow size distribution of the prepared nanoparticles (40-90 nm) with ~88 % encapsulation efficiency.