Development and validation of a sensitive LC-MS/MS method for pioglitazone: application towards pharmacokinetic and tissue distribution study in rats.

In the present study, a sensitive LC-MS/MS method was developed and validated to measure pioglitazone (PGZ) concentrations in rat plasma and tissues. The chromatographic separation was achieved by using a YMC Pro C column (100 mm × 4.6 mm, 3μ) with a mobile phase consisting of formic acid (0.

Quantification of rosiglitazone in rat plasma and tissues via LC-MS/MS: Method development, validation, and application in pharmacokinetic and tissue distribution studies.

A bioanalytical method for the quantification of rosiglitazone in rat plasma and tissues (adipose tissue, heart, brain, bone, and kidney) using LC-MS/MS was developed and validated. Chromatographic separation was achieved on a Gemini C column (50 × 4.6 mm, 3 μm) using a mobile phase consisting of 10 mM ammonium formate (pH 4.

A systematic approach for stability-indicating HPLC method optimization for Nilotinib bulk through design of experiments: Application towards characterization of base degradation products by mass spectrometry.

A novel and reliable stability-indicating high-performance liquid chromatography method was developed using design of experiments. Under forced degradation conditions (hydrolysis, oxidative, photolytic and thermal) Nilotinib produced five major degradation products utilizing sodium hydroxide in base hydrolysis. The degradation products were separated by Hypersil ODS column (150×4.

Review of pesticide residue analysis in fruits and vegetables. Pre-treatment, extraction and detection techniques.

A wide variety of pesticides have been used in agriculture to increase the yield, quality and extend the storage life of crops. However, the use of pesticide has been increased now a day due to the ever-increasing population and rapid urbanization. The continuous uses of these pesticides have resulted in contamination of the environment, crops and also caused potential risk to human health.

A sensitive liquid chromatography-tandem mass spectrometry method for quantitative bioanalysis of fingolimod in human blood: Application to pharmacokinetic study.

A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of fingolimod in human blood. The analyte and internal standard fingolimod-d4 were extracted from 300 μl of human blood using protein precipitation coupled with solid-phase extraction method. The chromatographic separation was achieved with a Kinetex biphenyl column (100 × 4.

A stability-indicating LC-MS/MS method optimization for Pemetrexed through design of experiments: Identification and characterization of major oxidative degradation product.

A stability-indicating high-performance liquid chromatography method for Pemetrexed bulk was developed and validated as per the ICH guidelines by employing design of experiments methodology equipped with Box-Behnken design. The separation of Pemetrexed and its oxidative degradation product was achieved in isocratic elution mode with Zorbax C column (150 mm x 4.6 mm x5 μm) using 10 mM ammonium acetate (pH 4.

A simple sensitive UFLC-MS/MS method for the simultaneous quantification of artesunate, dihydroartemisinin and quercetin in rat plasma and its application to pharmacokinetic studies.

An ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous estimation of artesunate (ART), dihydroartemisinin (DHA, an active metabolite of ART) and quercetin (QRT) in rat plasma. The separation was achieved using a Zorbax C column (3 μm, 50 mm × 4.6 mm) as a stationary phase with a mobile phase of 0.

Multivariate response surface methodology assisted modified QuEChERS extraction method for the evaluation of organophosphate pesticides in fruits and vegetables cultivated in Nilgiris, South India.

An effective, simple and sensitive analytical method has been developed employing liquid chromatography coupled with tandem mass spectrometry and validated for estimation of five organophosphate pesticides at trace levels in six fruits and twelve vegetables. Plackett-Burman design and central composite design was used to screen and optimize the significant factors in modified QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method. The method evaluation was done by matrix-matched calibration with linearity ranging from 5 to 500 µg/L with a correlation coefficient more than 0.

Experimental design in pesticide extraction methods: A review.

The sample extraction process is considered as the essential step in the pesticide residue analysis, as it provides the base for the detection of the pesticides in trace level. Various factors need to be optimized during the extraction of pesticides due to the complexity of the matrix which is time-consuming and tedious. Therefore, the use of experimental design in the optimization process proves to be effective with minimum experiments and cost.