Waste bamboo-derived magnetically separable bamboo-activated carbon: from characterization to effective remediation of fluoride (F) ions from water.

An effective and affordable nanoadsorbent, magnetically separable magnetite-activated bamboo carbon (MABC), was obtained from waste bamboo biomass pyrolysis of bamboo chunks and the co-precipitation method using ferrous and ferric chloride as iron precursors. The synthesized nanosorbents were characterised using XRD, SEM, and DLS techniques to study the surface characteristics and morphology. Chemical composition, optical absorption, and magnetic properties were studied using FTIR spectroscopy, UV-vis spectroscopy, and VSM, respectively.

Investigate the efficacy of size exclusion chromatography for the isolation of extracellular vesicles from C. elegans.

Isolation of Extracellular Vesicles (EVs) has been done extensively in the past using ultracentrifugation, a recent shift has been observed towards precipitation, and exosome isolation kits. These methods often co-elute contaminants of similar size and density which makes their detection and downstream applications quite challenging. As well as the EV yield is also compromised in some methodologies due to aggregate formation.

Gene-editing, immunological and iPSCs based therapeutics for muscular dystrophy.

Muscular dystrophy is a well-known genetically heterogeneous group of rare muscle disorders. This progressive disease causes the breakdown of skeletal muscles over time and leads to grave weakness. This breakdown is caused by a diverse pattern of mutations in dystrophin and dystrophin associated protein complex.

FTIR based approach to study EnaC mechanosensory functions.

The pulmonary epithelial sodium ion channel (ENaC) is gaining importance for its sodium gating and mechanosensitive roles. The mechano functional studies on ENaC suggest direct molecular interactions between the ENaC protein with cytoskeleton microtubules and other extracellular matrix components. Also, in few mechanotransduction studies, ENaC was shown to respond both to membrane stretch as well as cell volume changes.

Midgut aminopeptidase N expression profile in castor semilooper () during sublethal Cry toxin exposure.

The midgut of lepidopteran larvae is a multifunctional tissue that performs roles in digestion, absorption, immunity, transmission of pathogens and interaction with ingested various molecules. The proteins localized at the inner apical brush border membrane are primarily digestive proteases, but some of them, like aminopeptidase N, alkaline phosphatase, cadherins, ABC transporter C2, etc., interact with Crystal (Cry) toxins produced by ().

Midgut de novo transcriptome analysis and gene expression profiling of Achaea janata larvae exposed with Bacillus thuringiensis (Bt)-based biopesticide formulation.

India is the major producer and exporter of castor oil in the world. Castor semilooper, Achaea janata is one of the main castor crop pests, which causes serious economic loss of crop, hence management and control of the pest are important. Currently, Bacillus thuringiensis (Bt) based biopesticides are being used for their control.

Larval Mid-Gut Responses to Sub-Lethal Dose of Cry Toxin in Lepidopteran Pest .

The lack of homogeneity in field application of formulation often results in ingestion of sub-lethal doses of the biopesticide by a fraction of pest population and there by promotes the toxin tolerance and resistance in long term. Gut regeneration seems to be one of the possible mechanism by which this is accomplished. However, the existing information is primarily derived from studies using mid-gut cell cultures.

Insecticidal Effects of Hemocoelic Delivery of Cry Toxins in Larvae.

Insecticidal effects of Cry toxins in hemocoel of larvae have not been properly evaluated. In the present study, hemocoelic injection of four representative Cry toxins i.e.