[Some properties of plasmocoagulase of the causative agents of glanders and melioidosis].

Criteria for the evaluation of the plasmocoagulase activity of natural isolates and mutant strains of the causative agents of glanders and melioidosis were worked out, which made it possible to subdivide them by this sign into pathogens with high, moderate and low activity. Plasmocoagulase produced by pathogenic Burkholderia was shown to be a thermolabile enzyme, comparatively stable with respect to the action of such chemico-biological agents as hydrogen peroxide and chloramine.

[The enzyme-antienzyme interactions of a number of Burkholderia pseudomallei hydrolases with monoclonal immunoglobulins].

Phosphatase, phospholipase C and a proteolytic complex, including casein- and hemoglobin-hydrolases, have been isolated from cell-free extracts of B. pseudomallei cultivation medium. A set of monoclonal antibodies (McAb) to the antigenic complexes of this infective agent has been obtained.

[Thermostable Pseudomonas pseudomallei hemolysin].

The chemical nature of thermostable P.pseudomallei hemolysin has been determined. This substance is glycolipid, synthesized by bacteria at the stationary phase of growth under the conditions of the prevalence of the source of carbon over the source of nitrogen in the nutrient medium.

[The properties of the extracellular alkaline phosphatase of the causative agent of melioidosis].

After growing P. pseudomallei VPA on solid medium extracellular alkaline phosphatase with a molecular weight of 93,000 AMU was isolated, and practically purified from the extract of this medium by precipitation with ammonium sulfate, subsequent gel chromatography and concentration on membrane filters. The optimum conditions for enzymatic reaction were found to be pH 9.

[Use of ganglioside-containing magnetic polyacrylamide sorbents for the immunoenzyme method of determining cholera enterotoxin].

A variant of EIA techniques for the determination of cholera enterotoxin is proposed. This method is based on the selective sorption of the toxin on ganglioside-containing magnetic granules with its subsequent detection by means of immune serum and antispecific immunoperoxidase conjugate. The proposed method permits the detection of 0.

[Production of an erythrocytic ganglioside diagnostic reagent for detecting cholera enterotoxin and toxoid in indirect hemagglutination tests].

The studies described in this work have indicated that cholera enterotoxin and its components (cholerogenoid and subunit B) can be detected in amounts of 0.25, 0.28 and 0.

Immunobiological characteristic of antigens from the parasitic phase of Coccidiodes immitis.

The possibility of obtaining serologically active antigens from tissue spherules extracted from the lungs of albino mice infected with C. immitis has been demonstrated. Unlike the method of obtaining spherules in vitro, the accumulation of sufficient quantities of tissue spherules does not involve special difficulties.