Interaction of sp. RIT 592 induces the production of broad-spectrum antibiotics in sp. RIT 594.

Antimicrobial resistance (AMR) is one of the most alarming global public health challenges of the 21st century. Over 3 million antimicrobial-resistant infections occur in the United States annually, with nearly 50,000 cases being fatal. Innovations in drug discovery methods and platforms are crucial to identify novel antibiotics to combat AMR.

Whole-Genome Sequence of Endophytic Bacteria Associated with Poison Ivy Vine (Toxicodendron radicans).

Here, we report the genome assemblies of 11 endophytic bacteria, isolated from poison ivy vine (Toxicodendron radicans). Five species belonging to the genus Pseudomonas, two species of , one strain of Pantoea agglomerans, and one species from the , , and Enterobacter genera were isolated from the interior tissue of poison ivy.

Exiguobacterium sp. is endowed with antibiotic properties against Gram positive and negative bacteria.

Objective: In order to isolate and identify bacteria that produce potentially novel bactericidal/bacteriostatic compounds, two ponds on the campus of the Rochester Institute of Technology (RIT) were targeted as part of a bioprospecting effort.

Results: One of the unique isolates, RIT 452 was identified as Exiguobacterium sp. and subjected to whole-genome sequencing.

Whole-Genome Sequencing and Annotation of 10 Endophytic and Epiphytic Bacteria Isolated from Lolium arundinaceum.

We report the whole-genome sequence and annotation of 10 endophytic and epiphytic bacteria isolated from the grass as part of a laboratory exercise in a Fundamentals of Plant Biochemistry and Pathology undergraduate course (BIOL403) at the Rochester Institute of Technology in Rochester, New York.

Genomic characterization of bacteria from the ultra-oligotrophic Madison aquifer: insight into the archetypical LuxI/LuxR and identification of novel LuxR solos.

Objectives: To characterize the bacterial community of Wind Cave's Madison aquifer through whole-genome sequencing, and to better understand the bacterial ecology by identifying genes involved in acyl-homoserine lactone (AHL) based quorum-sensing (QS) systems.

Results: Genome-based taxonomic classification revealed the microbial richness present in the pristine Madison aquifer. The strains were found to span eleven genera and fourteen species, of which eight had uncertain taxonomic classifications.

Exploration of the Resistome Reveals Resistance Mechanisms in Commensals That May Be Acquired by through Horizontal Gene Transfer.

Nonpathogenic transfer mutations encoding antibiotic resistance to their pathogenic relative . However, the resistance genotypes and subsequent phenotypes of nonpathogens within the genus have been described infrequently. Here, we characterize the minimum inhibitory concentrations (MICs) of a panel of ( = 26)-including several commensal species-to a suite of diverse antibiotics.

Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria, RIT668 and RIT669, Isolated from Endangered Turtles ().

RIT668 and RIT669 were isolated from endangered spotted turtles (). Whole-genome sequencing, annotation and phylogenetic analyses of the genomes revealed that the closest relative of RIT668 is ATCC 7966 and A60 for RIT669. Resistome analysis showed that and harbor six and 19 different antibiotic resistance genes, respectively.

Isolation and whole-genome sequencing of Pseudomonas sp. RIT 623, a slow-growing bacterium endowed with antibiotic properties.

Objective: There is an urgent need for the discovery and/or development of novel antibiotics. We report an exploration of "slow"-growing bacteria, which can be difficult to isolate using rich media as they are usually outcompeted by "fast"-growing bacteria, as potential sources of novel antimicrobials.

Results: Pseudomonas sp.

Isolation and Whole-Genome Sequencing of 12 Mushroom-Associated Bacterial Strains: an Inquiry-Based Laboratory Exercise in a Genomics Course at the Rochester Institute of Technology.

Here, we report the isolation, identification, and whole-genome sequences of 12 bacterial strains associated with four mushroom species. The study was done as an inquiry-based exercise in an undergraduate genomics course (BIOL 340) in the Thomas H. Gosnell School of Life Sciences at the Rochester Institute of Technology.

Isolation, Whole-Genome Sequencing, and Annotation of sp. RIT 621, a Strain That Produces Antibiotic Compounds against Escherichia coli ATCC 25922 and Bacillus subtilis BGSC 168.

Here, we report the isolation, identification, whole-genome sequencing, and annotation of the bacterium sp. strain RIT 621. Concentrated spent medium extract treated with ethyl acetate was found to produce bactericidal compounds against the Gram-positive bacterium BGSC 168 and the Gram-negative bacterium ATCC 25922.

SELfies and CELLfies: Whole Genome Sequencing and Annotation of Five Antibiotic Resistant Bacteria Isolated from the Surfaces of Smartphones, An Inquiry Based Laboratory Exercise in a Genomics Undergraduate Course at the Rochester Institute of Technology.

Are touchscreen devices a public health risk for the transmission of pathogenic bacteria, especially those that are resistant to antibiotics? To investigate this, we embarked on a project aimed at isolating and identifying bacteria that are resistant to antibiotics from the screens of smartphones. Touchscreen devices have become ubiquitous in society, and it is important to evaluate the potential risks they pose towards public health, especially as it pertains to the harboring and transmission of pathogenic bacteria that are resistant to antibiotics. Sixteen bacteria were initially isolated of which five were unique (four species and one species).

Whole-Genome Sequencing and Annotation of Exiguobacterium sp. RIT 452, an Antibiotic-Producing Strain Isolated from a Pond Located on the Campus of the Rochester Institute of Technology.

Exiguobacterium sp. RIT 452 is of biotechnological importance given its potential for antibiotic production. Bactericidal activity was detected using spent medium extract in a disk diffusion assay against Escherichia coli.

Isolation and genomic characterization of six endophytic bacteria isolated from sp (sugarcane): Insights into antibiotic, secondary metabolite and quorum sensing metabolism.

Six endophytic bacteria were isolated from sp (sugarcane) grown in the parish of Westmoreland on the island of Jamaica located in the West Indies. Whole genome sequence and annotation of the six bacteria show that three were from the genus and the other three were from the genera , , and . A scan of each genome using the antibiotics and secondary metabolite analysis shell (antiSMASH4.