Dichotomous effects on lymphatic transport with loss of caveolae in mice.

Aim: Fluid and macromolecule transport from the interstitium into and through lymphatic vessels is necessary for tissue homeostasis. While lymphatic capillary structure suggests that passive, paracellular transport would be the predominant route of macromolecule entry, active caveolae-mediated transcellular transport has been identified in lymphatic endothelial cells (LECs) in vitro. Caveolae also mediate a wide array of endothelial cell processes, including nitric oxide regulation.

Cellular Origins of Beige Fat Cells Revisited.

Activated beige adipocytes have therapeutic potential due to their ability to improve glucose and lipid homeostasis. To date, the origin of beige adipocytes remains enigmatic. Whether beige cells arise through de novo differentiation from resident precursors or through reprogramming of mature white adipocytes has been a topic of intense discussion.

De novo adipocyte differentiation from Pdgfrβ preadipocytes protects against pathologic visceral adipose expansion in obesity.

Pathologic expansion of white adipose tissue (WAT) in obesity is characterized by adipocyte hypertrophy, inflammation, and fibrosis; however, factors triggering this maladaptive remodeling are largely unknown. Here, we test the hypothesis that the potential to recruit new adipocytes from Pdgfrβ preadipocytes determines visceral WAT health in obesity. We manipulate levels of Pparg, the master regulator of adipogenesis, in Pdgfrβ precursors of adult mice.

Fetal development of subcutaneous white adipose tissue is dependent on Zfp423.

Objective: is a multi zinc-finger transcription factor expressed in preadipocytes and mature adipocytes . Our recent work has revealed a critical role for in maintaining the fate of white adipocytes in adult mice through suppression of the beige cell thermogenic gene program; loss of in mature adipocytes of adult mice results in a white-to-beige phenotypic switch. However, the exact requirements of in the fetal stages of early adipose development have not been clarified.