2,3-Dihydroxybenzoate meta-Cleavage Pathway is Involved in o-Phthalate Utilization in Pseudomonas sp. strain PTH10.

Pseudomonas sp. strain PTH10 can utilize o-phthalate which is a key intermediate in the bacterial degradation of some polycyclic aromatic hydrocarbons. In this strain, o-phthalate is degraded to 2,3-dihydroxybenzoate and further metabolized via the 2,3-dihydroxybenzoate meta-cleavage pathway.

Isolation and characterization of a bacterial strain that degrades cis-dichloroethenein the absence of aromatic inducers.

Bacteria capable of degrading cis-dichloroethene (cDCE) were screened from cDCE-contaminated soil, and YKD221, a bacterial strain that exhibited a higher growth on minimal salt agar plates in the presence of cDCE than in the absence of cDCE, were isolated. Phylogenetic studies of the 16S rRNA as well as gyrB, rpoD, and recA in YKD221 indicated that this strain is closely related to the type strains of Pseudomonas plecoglossicida, monteilii, and putida. An average nucleotide identity analysis indicated that YKD221 is most closely related to P.

Identification of novel extracellular protein for PCB/biphenyl metabolism in Rhodococcus jostii RHA1.

Rhodococcus jostii RHA1 (RHA1) degrades polychlorinated biphenyl (PCB) via co-metabolism with biphenyl. To identify the novel open reading frames (ORFs) that contribute to PCB/biphenyl metabolism in RHA1, we compared chromatin immunoprecipitation chip and transcriptomic data. Six novel ORFs involved in PCB/biphenyl metabolism were identified.

γ-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.

The Rhodococcus jostii RHA1 gene cluster required for γ-resorcylate (GRA) catabolism was characterized. The cluster includes tsdA, tsdB, tsdC, tsdD, tsdR, tsdT, and tsdX, which encode GRA decarboxylase, resorcinol 4-hydroxylase, hydroxyquinol 1,2-dioxygenase, maleylacetate reductase, an IclR-type regulator, a major facilitator superfamily transporter, and a putative hydrolase, respectively. The tsdA gene conferred GRA decarboxylase activity on Escherichia coli.

Essential thrombocythemia as a risk factor for stillbirth.

Introduction: The risk of abortion is known to be high in women with essential thrombocythemia (ET). However, a few studies have focused on the risk of stillbirth among fetuses reaching gestational age compatible with life.

Methods: Review of medical charts of pregnant women with ET who received cares at a single center between January 2003 and June 2013 and the English literature in which more than 20 pregnancies with ET were dealt with regarding outcomes.

Difference in the D-dimer rise between women with singleton and multifetal pregnancies.

Introduction: The differences in the d-dimer rise between women with singleton and multifetal pregnancies have not been studied extensively.

Materials And Methods: d-Dimer levels were determined in 1089 blood specimens from 1089 women in various stages of pregnancy, including 977 and 112 women with singleton and multifetal pregnancies, respectively. None of the 1089 women developed hypertension or clinical venous thromboembolism during pregnancy or in the postpartum period.

Antithrombin activity, platelet count, hemoglobin concentration and hematocrit value determined immediately before vaginal delivery among healthy women.

Aims: To determine the normal reference values for antithrombin (AT) activity, platelet count (Plt), hemoglobin concentration (Hb), and hematocrit value (Ht) immediately before vaginal delivery among healthy pregnant women with singleton pregnancies and to determine association of these blood parameters with fetal growth.

Methods: A complete blood count was performed and the AT activity was examined in 300 consecutive women admitted to hospital at > or = gestational week 36 for labor pains and/or the rupture of fetal membranes. All the women were normotensive and had singleton pregnancies, and none of the women had proteinuria, a weekly weight gain > or = 0.

Identification and characterization of uptake systems for glucose and fructose in Rhodococcus jostii RHA1.

Rhodococcusjostii RHA1 takes up and utilizes glucose and fructose as its sole carbon source. To address glucose and fructose transport systems in RHA1, an in silico analysis was performed. BLAST searches revealed that the ro02365 and ro06844 genes share 51 and 47% amino acid identity, respectively, with the major facilitator superfamily (MFS) glucose transporter, GlcP, of Streptomycescoelicolor A3(2) and the ro01549-01552 and ro06781-06785 loci share 43-51% amino acid identity with proteins of the phosphotransferase system (PTS) in S.

Glucose-mediated transcriptional repression of PCB/biphenyl catabolic genes in Rhodococcus jostii RHA1.

Rhodococcus jostii RHA1, a Gram-positive polychlorinated biphenyl (PCB) degrader, exhibited biphasic growth in a medium containing biphenyl (BPH) and glucose (Glc), with consumption of Glc and low 2,3-dihydroxybiphenyl 1,2-dioxygenase activity in the first growth stage. These results suggested the repression of BPH metabolism in the presence of Glc, in which RHA1 preferentially utilized Glc in the first growth stage and BPH in the second stage. A reporter assay using a transcriptional fusion of the bphAa promoter (P(bphAa)) and the luxAB luciferase genes was performed, and lower luciferase activity was observed during the first growth stage, indicating transcriptional repression of P(bphAa) activity in the presence of Glc.

Do uterotrophic drugs increase the risk of fatal hemorrhagic brain stroke?

Objective: To evaluate whether uterotrophic agents increase the risk of fatal hemorrhagic brain stroke.

Methods: Between 1991 and 1992, there were 230 maternal deaths among 2,420,000 pregnant women in Japan and the causes of these deaths was investigated in 1994. Using information provided in this report, we identified 35 women who died from or were assumed to die from hemorrhagic brain stroke.

Evidence of the escape of antithrombin from the blood into the interstitial space in pregnant women.

Objective: we investigated whether ascites samples obtained from pregnant women during cesarean sections contained antithrombin because it is unknown whether antithrombin escapes from the blood and passes into the interstitial space during pregnancy.

Methods: the concentration and activity levels of antithrombin were determined in six ascites samples obtained from six consecutive women who exhibited generalized edema, ascites, and a gradual decline in antithrombin activity.

Results: all six ascites samples contained antithrombin (mean ± SD, 4.

Marked gestational edema as a clinical sign of life-threatening condition.

A 35-year-old Japanese nulliparous woman exhibited rapid weight gain (6 kg/7 days), reduced antithrombin activity and platelet count at 37 weeks of gestation without hypertension or proteinuria, and underwent cesarean section. Postnatally, pulmonary edema developed for 7 days, with transient hypertension and proteinuria, and bodyweight loss (14.6 kg) by 14 days postpartum.

[Successful desensitization using cisplatin after carboplatin-associated hypersensitivity reactions in 3 cases with recurrent ovarian carcinoma].

The standard chemotherapy for ovarian carcinoma is paclitaxel and carboplatin concomitant therapy. This combination is so frequently administered that a carboplatin-associated hypersensitivity reaction may occur after long-term treatment. Recently, we experienced carboplatin-associated hypersensitivity reactions in three cases.

The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse.

Rhodococcus sp. RHA1 (RHA1) is a potent polychlorinated biphenyl-degrading soil actinomycete that catabolizes a wide range of compounds and represents a genus of considerable industrial interest. RHA1 has one of the largest bacterial genomes sequenced to date, comprising 9,702,737 bp (67% G+C) arranged in a linear chromosome and three linear plasmids.