2'-anthraquinone-conjugated oligonucleotide as an electrochemical probe for DNA mismatch.

We previously prepared the oligonucleotides (ODNs) conjugated to an anthraquinone (AQ) group via one carbon linker at the 2'-sugar position. When these modified ODNs bind to cDNA sequences, the AQ moiety can be intercalated into the predetermined base-pair pocket of a duplex DNA. In this paper, 2'-AQ-modified ODNs are shown to be an excellent electrochemical probe to clarify the effect of a mismatch base on the charge transfer (CT) though DNA.

Electrochemistry of redox-modified oligonucleotides bound to gold surface.

Electrochemical properties of redox-active DNA bound to gold surface were described.

Evidence for the transglycosylation of complex type oligosaccharides of glycoproteins by endo-beta-N-acetylglucosaminidase HS.

Transglycosylation activity of endo-beta-N-acetylglucosaminidase HS (Endo HS) was investigated using native human transferrin as a donor of an asparagine-linked oligosaccharide and p-nitrophenyl-beta-d-glucose (PNP-beta-d-Glc) as an acceptor of the oligosaccharide. The amount of the product increased dependent on the concentration of the acceptors. Absorption spectrum, exoglycosidase digestion and matrix assisted laser desorption and ionization-time of flight (MALDI-TOF) mass analysis of the transglycosylation product indicated that the asialobiantennary complex type oligosaccharide of human transferrin was transferred to PNP-beta-d-Glc.

Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2.

Acetylcarnitine exerts therapeutic effects on some neurological disorders including Alzheimer's disease. OCTN2 is known as a transporter for acetylcarnitine, but its expression in the brain is very low. To examine a brain-specific transporter for acetylcarnitine, we screened a rat brain cDNA library by hybridization using a DNA probe conserved among an OCTN family.

Electrochemical detection of single-base mismatches in DNA by a redox-active intercalator conjugated oligonucleotide.

A simple and direct electrochemical detection of DNA single-base mismatches utilizing anthraquinone-modified oligonucleotides (AQ-ODNs) bound to gold electrodes has been described. By using the redox-active oligonucleotides, the redox-active center can be positioned at the desired base-pair pocket in double-helical DNA, enabling us to measure the charge transport between the redox-active center and the electrode that sandwich mismatched and full-matched base-pairs in double helix. AQ-ODNs immobilized on gold electrodes have been found to be useful in the electrochemical discrimination of hybrids containing a single-base mismatch from the one with fully matched bases.

Probing DNA sequences by anthraquinone-modified DNA that is immobilized on gold surface.

2'-Anthraquinone-modified oligonucleotide (AQ-ODN) possessing disulfide terminus has been immobilized on the gold electrode surface. The AQ-ODN-modified electrode showed faster electron transferability in double-stranded form than that in single-stranded form.