Decellularizing corneal stroma using N2 gas.

Purpose: To examine the efficacy of a novel method of decellularizing porcine corneal stroma using N(2) gas from liquid N(2) and the feasibility of using decellularized porcine corneal stroma in a corneal transplantation model in rabbits.

Methods: Porcine corneas were placed in a tube, and N(2) gas from liquid N(2) was poured into the tube to freeze the corneas and make the inside of the tube hypoxic. After fastening the cap firmly, the tube was kept at room temperature for seven days, and the porcine corneas were examined histologically.

New fava bean guard cell signaling mutant impaired in ABA-induced stomatal closure.

We isolated a mutant from Vicia faba L. cv. House Ryousai.

Antigenicity of porcine cornea as xenograft.

Purpose: To investigate the antigenicity of porcine corneal stroma as xenograft to man.

Methods: The localization of alpha-gal epitope in the porcine eye was determined using biotinylated Griffonia simplicifolia 1 isolectin B4. Porcine corneal stromal was inserted into corneal stromal pockets of cynomolgus monkeys.

Magnetic attraction of iron-endocytosed corneal endothelial cells to Descemet's membrane.

Purpose: To evaluate the feasibility of a novel method of magnetic attraction of iron-endocytosed corneal endothelial cells to Descemet's membrane.

Methods: Cultured rabbit corneal endothelial cells (RCEC) were exposed to spherical iron powder at various concentration ranging 0-100 micro moll(-1). After 24hr, the cell density and morphology were evaluated.