Publications by authors named "Nao Fukuda"
Article Synopsis
- The study aimed to examine how PEGylation affects the uptake of the osteoprotegerin/osteoclastogenesis inhibitory factor (OCIF) in rat and human liver, kidney, and spleen.
- Rats were given a PEGylated version of OCIF, and researchers measured the tissue concentrations after 15 minutes, while human liver uptake was tested through liver perfusion with both OCIF and a labeled version of the PEGylated OCIF.
- Results showed that the PEGylated OCIF had significantly lower tissue uptake in both rats and human liver compared to the non-PEGylated form, suggesting that PEGylation could enhance the therapeutic potential of OCIF in medical applications.
To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolated in 2003.
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