Publications by authors named "Nanna Monje"
Background: The aim of this study was to analyse transcriptomic differences between primary and recurrent high-grade serous ovarian carcinoma (HGSOC) to identify prognostic biomarkers.
Methods: We analysed 19 paired primary and recurrent HGSOC samples using targeted RNA sequencing. We selected the best candidates using in silico survival and pathway analysis and validated the biomarkers using immunohistochemistry on a cohort of 44 paired samples, an additional cohort of 504 primary HGSOCs and explored their function.
Article Synopsis
- * In a study of 571 HGSC patients, higher levels of LRP1B in tumor and surrounding stromal cells were found to significantly extend overall survival (OS) and progression-free survival (PFS) by up to 42 and 19 months, respectively.
- * The findings suggest that LRP1B could serve as a valuable prognostic marker and help explore lipid metabolism's role in advanced HGSC, potentially influencing treatment strategies like liposomal therapies.
Background: The enzyme indoleamine 2,3-dioxygenase 1 (IDO1) plays a crucial role in regulating the immune system's response to tumors, but its exact role in cancer, especially in high-grade serous ovarian cancer (HGSOC), remains controversial. We aimed to investigate the prognostic impact of IDO1 expression and its correlation with tumor-infiltrating lymphocytes (TILs) in HGSOC.
Methods: Immunohistochemical (IHC) staining and bioimage analysis using the QuPath software were employed to assess IDO1 protein expression in a well-characterized cohort of 507 patients with primary HGSOC.
Article Synopsis
- RGS2, a regulator of G-protein signaling, has been linked to poor prognosis in high-grade serous ovarian carcinoma (HGSOC), making it a potential target for new therapies.
- In a study involving 519 patients, RGS2 showed low levels of expression at the mRNA level but varying levels of protein expression, with low protein levels correlating with worse overall and progression-free survival.
- The research highlights the need for further exploration of RGS2's functional role in HGSOC, especially given its promising implications for drug development.
Purpose: In recent years the tumor microenvironment and its interaction with the tumor has emerged into research focus with increased attention to the composition of Tumor-infiltrating lymphocytes. We wanted to quantify the composition of Regulatory T cells (Tregs) and T helper 17 cells (Th17 cells) and their prognostic impact in high-grade serous tubo-ovarian carcinoma.
Methods: Tregs and Th17 cells were determined by immunohistochemical analysis of CD25 FoxP3 and RORγt, respectively on tissue microarrays of a cohort of 222 patients with reviewed histology and available clinical data.
Background: Pancreatic neuroendocrine neoplasms (PanNENs) fall into two subclasses: the well-differentiated, low- to high-grade pancreatic neuroendocrine tumors (PanNETs), and the poorly-differentiated, high-grade pancreatic neuroendocrine carcinomas (PanNECs). While recent studies suggest an endocrine descent of PanNETs, the origin of PanNECs remains unknown.
Methods: We performed DNA methylation analysis for 57 PanNEN samples and found that distinct methylation profiles separated PanNENs into two major groups, clearly distinguishing high-grade PanNECs from other PanNETs including high-grade NETG3.