4-Methyl-N-.

The title compound, C(20)H(19)NO(2)S(2), is formed by a palladium-copper-catalyzed reaction between 4-methyl-N-[2-(prop-2-ynylsulfanyl)phenyl]benzenesulfonamide and p-iodotoluene. The molecules contain three essentially planar parts, namely an aminothiophenol moiety (A), a toluenesulfone moiety excluding the oxo ligands (B) and a tolyl group (C), approximately orthogonal to each other; the dihedral angles A/B, A/C and B/C are 111.6 (1), 89.

Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW.

The distribution of genes for an outer membrane protein (OmpW) and a regulatory protein (ToxR) in Vibrio cholerae and other organisms was studied using respective primers and probes. PCR amplification results showed that all (100%) of the 254 V. cholerae strains tested were positive for ompW and 229 ( approximately 98%) of 233 were positive for toxR.

Three-pion interferometry results from central Pb+Pb collisions at 158A GeV/c.

Three-particle correlations have been measured for identified pi(-) from central 158A GeV Pb+Pb collisions by the WA98 experiment at CERN. A substantial contribution of the genuine three-body correlation has been found as expected for a mainly chaotic and symmetric source.

(E)-2-[2-(1-Naphthyl)vinyl]-3-tosyl-2,3-dihydro-1,3-benzothiazole.

The title compound, C(26)H(21)NO(2)S(2), which consists of a benzothiazole skeleton with alpha-naphthylvinyl and tosyl groups at positions 2 and 3, respectively, was prepared by palladium-copper-catalyzed heteroannulation. The E configuration of the molecule about the vinyl C=C bond is established by the benzothiazole-naphthyl C-C-C-C torsion angle of 177.5 (4) degrees.

Heteroannulation through copper catalysis: a novel cyclization leading to a highly regio- and stereoselective synthesis of 2-substituted benzothiazolines.

[reaction: see text] 3-(2-Aminophenylthio)prop-1-yne 8 reacted with aryl iodides 9-18 under palladium-copper catalysis leading to the disubstituted alkynes 19-28 which after tosylation underwent a novel cyclization under copper catalysis to 2-substituted benzothiazolines 29-38. The expected 3-(arylidine)-2,3-dihydrobenzothiazines were not obtained.

Antibodies (IgG) to lipopolysaccharide of Vibrio cholerae O1 mediate protection through inhibition of intestinal adherence and colonisation in a mouse model.

An antiserum raised against purified lipopolysaccharide (LPS) of a Vibrio cholerae O1 strain (Co366) induced passive protection against challenge with the parent as well as other O1 organisms but not against O139 or non-O1/non-O139 organisms. A considerable level of protection against O1 strains was also observed with the IgG fraction of the antiserum which inhibited intestinal adherence and colonisation. The monovalent Fab(IgG) fragment, on the other hand, showed only a low level of protection.

Molecular characterization of a new variant of toxin-coregulated pilus protein (TcpA) in a toxigenic non-O1/Non-O139 strain of Vibrio cholerae.

A toxigenic non-O1/non-O139 strain of Vibrio cholerae (10259) was found to contain a new variant of the toxin-coregulated pilus (TCP) protein gene (tcpA) as determined by PCR and Southern hybridization experiments. Nucleotide sequence analysis data of the new tcpA gene in strain 10259 (O53) showed it to be about 74 and 72% identical to those of O1 classical and El Tor biotype strains, respectively. The predicted amino acid sequence of the 10259 TcpA protein shared about 81 and 78% identity with the corresponding sequences of classical and El Tor TcpA strains, respectively.

A CLINICAL STUDY OF FALCIPARUM MALARIA - 302 CASES.

Three hundred and two cases of falciparum malaria including 30 cases of severe falciparum malaria admitted between Jan 93 - Dec 93 were studied for their clinical features and response to treatment. Intermittent or remittent fever with chills and headache were the commonest presentation noted (99.3%).

Subcellular localization of IpaC, an invasion plasmid antigen of Shigella dysenteriae type 1 and its in-vitro binding capability to mammalian cells.

Invasion plasmid antigen C (IpaC), a 45-kDa protein encoded by an invasion plasmid of Shigella, is associated with the invasion of epithelial cells by the bacteria. Invasive strains of S. dysenteriae type 1 secreted more proteins into the extracellular environment than a non-invasive strain and secreted more IpaC protein.

A new mycotoxin from Aspergillus candidus Link isolated from rough rice.

A new mycotoxin (AcT1) was obtained from the mycelium of Aspergillus candidus Link isolated from rough rice stored under tropical conditions. AcT1 with the mol-formula C28H40O showed bright greenish blue fluorescence under uv (254 nm). The spectral and other characteristics indicated that the compound was a new one.

Acceptability and pathological changes in organs of rats fed wheat contaminated with a mixed culture of Aspergillus and Penicillium species.

Mouldy grains can cause problems with pathological changes in vital organs. This investigation involved histological studies including the occurrence of fungal conidiospores in organs of rats fed wheat contaminated by the addition of a mixed culture of Aspergillus and Penicillium spp.

Plasma concentrations of thioridazine metabolites and ECG abnormalities.

Five acute schizophrenic patients were given a standard thioridazine dose (4 mg/kg/day po). Their plasma concentrations of thioridazine and its metabolites were determined by GLC, and their ECG's were recorded routinely. In four of the five patients, abnormal ECG's (malformation of the T-wave or lengthening of the repolarization time) were found whenever plasma concentrations of the thioridazine ring sulfoxide were elevated.

Iron and hemoglobin status of the offspring of guinea pigs whose mother received large doses of vitamin C during pregnancy and lactation.

1. Lower level of serum and liver iron were noted in the offspring of experimental group quinea pigs who received 1, 2 and 3 mg ascorbic acid per 100 g body weight per day. 2.

Effects of high intake of vitamin C by the guinea pigs in pregnancy and lactation on the tissue levels of the vitamin in their offspring.

1. Though the growth-rate of the guinea pigs of both the groups - control and experimental were almost same, the gain in body weight in the offspring of experimental group animals were observed to be less when they were fed 1, 2 and 3 mg ascorbic acid per 100 g body weight per day. 2.

GLC analysis of thioridazine, mesoridazine, and their metabolites.

A GLC method for measuring thioridazine, mesoridazine, their metabolites, and possibly other phenothiazines was developed. By using this method, seven different phenothiazine derivatives, thioridazine, and six known thioridazine metabolites were extracted and separated. This method was tested by assaying plasma samples from 30 hospitalized psychiatric patients receiving thioridazine or mesoridazine.

Effect of ascorbic acid on histamine metabolism in scorbutic guinea-pigs.

1. In guinea-pigs fed an ascorbic-acid-free diet, as the ascorbic acid levels decreased the histamine levels in blood and urine rose steadily to maxima in about 10-12 days. The elevated histamine levels persisted in the blood and urine of scorbutic guinea-pigs and the histamine levels in lung, gastric mucosa and spleen also increased.

Nutrient interrelationship of ascorbic acid and iron in rats and guinea pigs fed cereal diets.

Iron at a daily dose of 5 mg per animal was toxic to rats and guinea pigs fed unfortified whole wheat flour and whole rice flour diets. In rats, a species capable of synthesizing ascorbic acid, iron supplementation resulted only in decreased growth. In guinea pigs, iron administration resulted not only in decreased growth but also in high mortality.