Genome sequencing of cucumber mosaic virus (CMV) isolates infecting chilli and its interaction with host ferredoxin protein of different host for causing mosaic symptoms.

Unlabelled: Chilli ( L.) is an important vegetable crop grown in the Indian sub-continent and is prone to viral infections under field conditions. During the field survey, leaf samples from chilli plants showing typical symptoms of disease caused by cucumber mosaic virus (CMV) such as mild mosaic, mottling and leaf distortion were collected.

Single visit rehabilitation of 12-day neonate with cleft palate using modified feeding spoon impression technique: A case report.

The rehabilitation of the cleft requires a multidisciplinary approach involving a pediatrician, surgeon, otolaryngologist, speech therapist, orthodontist, prosthodontist, and psychologist. The present case report illustrates the rehabilitation of a 12-day-old neonate with a cleft palate. Since the palatal arch of the neonate was very small, a feeding spoon was innovatively modified to obtain the impression.

Two isoleucyl tRNAs that decode synonymous codons divergently regulate breast cancer metastatic growth by controlling translation of proliferation-regulating genes.

The human genome contains 61 codons encoding 20 amino acids. Synonymous codons representing a given amino acid are decoded by a set of transfer RNAs (tRNAs) called isoacceptors. We report the surprising observation that two isoacceptor tRNAs that decode synonymous codons become modulated in opposing directions during breast cancer progression.

Emergence and expansion of highly infectious spike protein D614G mutant SARS-CoV-2 in central India.

COVID-19 has emerged as global pandemic with largest damage to the public health, economy and human psyche.The genome sequence data obtained during the ongoing pandemic are valuable to understand the virus evolutionary patterns and spread across the globe. Increased availability of genome information of circulating SARS-CoV-2 strains in India will enable the scientific community to understand the emergence of new variants and their impact on human health.

Modified Retromastoid Approach and Clipping of "High-Riding" VA-PICA Junction Aneurysm: An Operative Video.

Background And Introduction: Clipping an aneurysm on an elongated and tented V4 segment near the origin of the posterior inferior cerebellar artery (high-riding VA-PICA junction aneurysm) can be challenging.

Objective: We demonstrate the microsurgical clipping technique of such an aneurysm using a modified retromastoid approach (MRMA) and glossopharyngeal-cochlear triangle (GCT).

Surgical Technique: A 50-year-old female with a ruptured high-riding left VA-PICA junction aneurysm underwent an MRMA.

Transmission, characterization and occurrence of recombination in Indian strain of associated with yellow mosaic and leaf curl disease of Summer squash.

Unlabelled: Summer squash is one of the important vegetable crops and its production is hampered by various abiotic and biotic stresses. Of the different biotic stresses, viral infections are responsible for causing great losses to this crop. Diseases caused by begomoviruses are becoming a major constraint in the cultivation of summer squash.

In silico docking analysis revealed the potential of phytochemicals present in and , used in Ayurveda medicine in inhibiting SARS-CoV-2.

Unlabelled: The 2 (SARS-CoV-2) has resulted in outbreak of global pandemic, fatal pneumonia in human referred as Coronavirus Disease-2019 (Covid-19). Ayurveda, the age old practice of treating human ailments in India, can be considered against SARS-CoV-2. Attempt was made to provide preliminary evidences for interaction of 35 phytochemicals from two plants ( and used in Ayurveda with SARS-CoV-2 proteins (open & closed state S protein, 3CLpro, PLpro and RdRp) through in silico docking analysis.

Source identification and management of perennial contaminated groundwater seepage in the highly industrial watershed, south India.

Perennial contaminated groundwater seepage is threatening the downstream ecosystem of the Kazipally Pharmaceutical industrial area located in South India. The sources of seepage are unknown for the last three decades that challenging the regulatory authorities and industries. In general, water quality monitoring and geophysical techniques are applied to identify the sources.

Potentially toxic elements (PTEs) pollution in surface soils in a typical urban region of south India: An application of health risk assessment and distribution pattern.

The pollution level of potentially toxic elements (PTEs) in surface soils is detrimental to the ecosystem and human health. In this research, various indices such as an index of geo-accumulation (I), contamination factor (CF), degree of contamination (DC), and principal component analysis (PCA) were implemented to identify and evaluate the soil PTEs pollution; and then human health risk assessment model used to establish the link between heavy metals pollution and human health in the urban region of south India. Results exhibited that the mean concentration of Cr, Cu, Ni and Zn were found to be 1.

Groundwater chemistry integrating the pollution index of groundwater and evaluation of potential human health risk: A case study from hard rock terrain of south India.

Groundwater is an important resource for drinking and irrigation purposes and also the significant route of human exposure in most of the arid and semi-arid regions of the world. In view of this, 43 groundwater samples were collected and analyzed for various physico-chemical parameters. Particularly, this study integrates the groundwater contamination by comparing it to national guidelines and the impact of fluoride and nitrate on health risk were quantified through the model recommended by the United States Environmental Protection Agency (USEPA).

Effect of Experiential Competency-Based Interprofessional Education on Pre-Professional Undergraduate Students: A Pilot Study.

The purpose of this pilot study was to evaluate the effectiveness of an interdisciplinary faculty team-led competency-based interprofessional undergraduate course for health and human services pre-professional students. Today's complex health and social problems require inter-professional knowledge and skills. Most interprofessional education occurs in graduate-level healthcare programs.

KLF5 mediates the hyper-proliferative phenotype of the intestinal epithelium in mice with intestine-specific endogenous K-Ras expression.

Oncogenic activation is a common mutational event in colorectal cancer. We previously showed that transcription factor, Krüppel-like factor 5 (KLF5), contributes to intestinal polyposis in mice with activation. At 14 months of age, mice developed small intestinal and colonic hyperplastic polyps while had none.

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses.

Understanding the role of factors that regulate intestinal epithelial homeostasis and response to injury and regeneration is important. The current literature describes several different methodological approaches to obtain images of intestinal tissues for data validation. In this paper, we delineate a common protocol relating to the derivation and processing of mouse intestinal tissues.

Role of Krüppel-like factor 5 in the maintenance of the stem cell niche in the intestinal crypt.

The intestinal epithelium is a tissue that undergoes continuous self-renewal initiated at the bottom of the crypts, which harbor the intestinal stem cell (ISC) pool. The ISC pool is sub-divided into crypt base columnar (CBC) cells at the crypt bottom and label retention cells (LRC) at position +4 from the crypt bottom. CBC cells are marked by Leucine-rich repeat-containing G-protein coupled receptor (Lgr5) while LRC cells are identified by several markers including Bmi1, mTert, Hopx, Lrig1, and Sox9.

Krüppel-like factor 6 regulates mitochondrial function in the kidney.

Maintenance of mitochondrial structure and function is critical for preventing podocyte apoptosis and eventual glomerulosclerosis in the kidney; however, the transcription factors that regulate mitochondrial function in podocyte injury remain to be identified. Here, we identified Krüppel-like factor 6 (KLF6), a zinc finger domain transcription factor, as an essential regulator of mitochondrial function in podocyte apoptosis. We observed that podocyte-specific deletion of Klf6 increased the susceptibility of a resistant mouse strain to adriamycin-induced (ADR-induced) focal segmental glomerulosclerosis (FSGS).

Transition from amplitude to oscillation death in a network of oscillators.

We report a transition from a homogeneous steady state (HSS) to inhomogeneous steady states (IHSSs) in a network of globally coupled identical oscillators. We perturb a synchronized population of oscillators in the network with a few local negative or repulsive mean field links. The whole population splits into two clusters for a certain number of repulsive mean field links and a range of coupling strength.

Krüppel-like factor 5 is essential for proliferation and survival of mouse intestinal epithelial stem cells.

Krüppel-like factor 5 (KLF5) is a pro-proliferative transcription factor that is expressed in dividing epithelial cells of the intestinal crypt. Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) has been identified as a stem cell marker in both small intestinal and colonic epithelial cells. To determine whether KLF5 regulates proliferation of intestinal stem cells, we investigated the effects of Klf5 deletion specifically from the intestinal stem cells in adult mice.

Krüppel-like factor 4 is a radioprotective factor for the intestine following γ-radiation-induced gut injury in mice.

Gut radiation-induced injury is a concern during treatment of patients with cancer. Krüppel-like factor 4 (KLF4) is expressed in differentiated villous epithelial cells of the small intestine. We previously showed that KLF4 protects cells from apoptosis following γ-irradiation in vitro.

Interprofessional practice and education: holistic approaches to complex health care challenges.

The incidence of chronic health conditions continues to rise worldwide. As a result, many groups and decision makers are calling for greater use of interprofessional health care teams and education to effectively address the social, psychological, biological, environmental, and economic dimensions inherent in these health care challenges. This article examines interprofessional practice (IPP) and interprofessional education (IPE) and addresses the following questions: (1) What is IPP; (2) What is IPE; (3) What competencies are necessary to effectively engage in IPP; (4) Who should be educated to serve on IPP health delivery teams; and (5) What educational model can best prepare health care professionals with the skills and practice experiences needed to address complex health issues in a comprehensive fashion? We propose a unique university-based IPE model that effectively addresses complex health issues and maximizes collaboration among clinical, nonclinical, and community stakeholders.

An innovative interprofessional education model to engage community and nonclinical participants.

Many institutions and individuals are encouraging use of interprofessional educational models to prepare future health care professionals who can effectively address complex healthcare needs in a dynamic healthcare environment. This report describes a seven-step model to plan and implement university-based interprofessional healthcare education. The model is unique in that it incorporates faculty and students from both clinical and nonclinical professional programs, administrators, as well as community partners into the interprofessional healthcare education deliberation and implementation process.

Inducible intestine-specific deletion of Krüppel-like factor 5 is characterized by a regenerative response in adult mouse colon.

Krüppel-like factor 5 (KLF5) is a pro-proliferative transcriptional regulator primarily expressed in the intestinal crypt epithelial cells. Constitutive intestine-specific deletion of Klf5 is neonatal lethal suggesting a crucial role for KLF5 in intestinal development and homeostasis. We have previously shown Klf5 to play an active role regulating intestinal tumorigenesis.

A colon cancer-derived mutant of Krüppel-like factor 5 (KLF5) is resistant to degradation by glycogen synthase kinase 3β (GSK3β) and the E3 ubiquitin ligase F-box and WD repeat domain-containing 7α (FBW7α).

Krüppel-like factor 5 (KLF5) is a zinc finger transcription factor that is highly expressed in the crypt epithelial cells of the intestine and plays a critical role in regulating proliferation of both normal intestinal epithelial cells and colorectal cancer cells. Stability of the KLF5 is mediated by proteasomal degradation via phosphorylation by glycogen synthase kinase 3β (GSK3β) and recognition by F-box and WD repeat domain-containing 7 (FBW7) of a phosphodegron sequence surrounding serine 303 in KLF5. A genomic analysis of colorectal cancer tissues identified a somatic mutation (P301S) in KLF5 within the phosphodegron sequence.