Analysis and Purification of Synthetic Nucleic Acids Using HPLC.

Chromatographic methods have been essential tools for analysis and purification of synthetic oligonucleotides since the 1970s. Significant developments in terms of instruments and stationary phases (media) have been made during the past several decades; among the latest are sub-micron to micron particles for the media, as well as ultra performance liquid chromatography (UPLC). Micron and sub-micron particles have increased product resolution.

Highly effective non-explosive activators based on saccharin for the synthesis of oligonucleotides and phosphoramidites.

A new class of non-explosive activators has been developed based on heterocyclic tertiary amine salts of saccharin. These salts have been found to be highly effective in the synthesis of oligonucleotides and nucleoside phosphoramidites.

Recent developments in the chemistry, analysis and control for the manufacture of therapeutic-grade synthetic oligonucleotides.

Significant developments in oligonucleotide chemistry, which occurred between 1980 and 2000, led to several oligonucleotide-based therapeutics entering clinical trials. Improvements in synthesis and purification methods, along with analytical techniques have facilitated the large-scale manufacture of high quality oligonucleotides to support these clinical trials.