Publications by authors named "Nancy da Rocha Torres Pavione"
Heterologous expression and biochemical characterization of the recombinant nucleoside triphosphate diphosphohydrolase 2 (LbNTPDase2) from Leishmania (Viannia) braziliensis.
Purinergic Signal
October 2024
Article Synopsis
- Leishmania braziliensis causes American Tegumentary Leishmaniasis (ATL), a significant tropical disease, and has two isoforms of the enzyme ENTPDase, which is linked to its infectivity.
- The study focused on the recombinant ENTPDase2 enzyme (rLbNTPDase2), showing it has apyrase activity, can break down various nucleotides, and needs divalent cations like calcium or magnesium for function.
- Characterization of rLbNTPDase2 revealed it works best at neutral to basic pH, is somewhat inhibited by certain compounds, and its low Km for ADP indicates it might be important for signaling processes, suggesting it could be a new
Synthesis of new non-natural L-glycosidic flavonoid derivatives and their evaluation as inhibitors of Trypanosoma cruzi ecto-nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase1).
Purinergic Signal
August 2024
Trypanosoma cruzi is the pathogen of Chagas disease, a neglected tropical disease that affects more than 6 million people worldwide. There are no vaccines to prevent infection, and the therapeutic arsenal is very minimal and toxic. The unique E-NTPDase of T.
View Article and Find Full Text PDFLateral flow immunochromatography is a widely used technique for immunological assays. Construction of test and control lines is mostly done by antigen adsorption to nitrocellulose membranes, a process not fully understood. This study aimed to evaluate the influence of urea, salts, and Tween 20, on adsorption.
View Article and Find Full Text PDFCanine visceral leishmaniasis (CVL) has been the theme of several studies given the importance of dog as natural reservoir of the pathogen Leishmania infantum in endemic regions and its role on dissemination of CVL and human visceral Lesihmaniasis (VL). The current immunodiagnosis of CVL has limitations concerning accuracy, specificity and sensitivity. Therefore, improvements are required.
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