Identification of quantitative trait loci for osteoarthritis of hip joints in dogs.

Objective: To identify quantitative trait loci (QTL) associated with osteoarthritis (OA) of hip joints of dogs by use of a whole-genome microsatellite scan.

Animals: 116 founder, backcross, F1, and F2 dogs from a crossbred pedigree.

Procedures: Necropsy scores and an optimized set of 342 microsatellite markers were used for interval mapping by means of a combined backcross and F2 design module from an online statistical program.

A random model for mapping imprinted quantitative trait loci in a structured pedigree: an implication for mapping canine hip dysplasia.

Genetic imprinting may have played a more notable role in shaping embryonic development of plants, animals, and humans than previously appreciated. Quantitative trait loci that are imprinted (iQTL) exert monoallelic effects, depending on the parent of origin, which is an exception to the laws of Mendelian genetics. In this article, we present a modified random effect-based mapping model to use in a genome-wide scan for the distribution of iQTL that contribute to genetic variance for a complex trait in a structured pedigree.

Quantitative genetics of secondary hip joint osteoarthritis in a Labrador Retriever-Greyhound pedigree.

Objective: To evaluate the quantitative inheritance of secondary hip joint osteoarthritis in a canine pedigree.

Animals: 137 Labrador Retrievers, Greyhounds, and mixed-breed dogs.

Procedures: Necropsy scores ranging from 0 to 4 were obtained for each hip joint.

Assessment of bone mineral density of the femoral head in dogs with early osteoarthritis.

Objective: To compare the bone mineral density (BMD) of the proximal portion of the femur in dogs with and without early osteoarthritis secondary to hip dysplasia.

Animals: 24 dogs (3 Greyhounds, 6 Labrador-Greyhound crossbreeds, and 15 Labrador Retrievers).

Procedure: Computed tomography (CT) of the pelvis, including a bone-density phantom, was performed for each dog.

Quantitative trait loci for hip dysplasia in a cross-breed canine pedigree.

Canine hip dysplasia is a common developmental inherited trait characterized by hip laxity, subluxation or incongruity of the femoral head and acetabulum in affected hips. The inheritance pattern is complex and the mutations contributing to trait expression are unknown. In the study reported here, 240 microsatellite markers distributed in 38 autosomes and the X chromosome were genotyped on 152 dogs from three generations of a crossbred pedigree based on trait-free Greyhound and dysplastic Labrador Retriever founders.

Evaluation of multiple radiographic predictors of cartilage lesions in the hip joints of eight-month-old dogs.

Objective: To determine the radiographic methods that best predict the development of osteoarthritis in the hip joints of a cohort of dogs with hip dysplasia and unaffected dogs.

Animals: 205 Labrador Retrievers, Greyhounds, and Labrador Retriever-Greyhound crossbred dogs.

Procedure: Pelvic radiography was performed when the dogs were 8 months old.

Effect of dorsal hip loading, sedation, and general anesthesia on the dorsolateral subluxation score in dogs.

Objective: To determine whether dorsal loading of the pelvis and type of chemical restraint affected the dorsolateral subluxation (DLS) score of dog hips. STYDY DESIGN: In vivo testing of diagnostic method.

Animals: Labrador retrievers, Greyhounds, and crosses between both breeds (n = 119 dogs).

Power of a Labrador Retriever-Greyhound pedigree for linkage analysis of hip dysplasia and osteoarthritis.

Objective: To estimate the number of dogs required to find linkage to heritable traits of hip dysplasia in dogs from an experimental pedigree.

Animals: 147 Labrador Retrievers, Greyhounds, and their crossbreed offspring.

Procedure: Labrador Retrievers with hip dysplasia were crossed with unaffected Greyhounds.

The cartilage-specific fibronectin isoform has a high affinity binding site for the small proteoglycan decorin.

Binding of fibronectin to the small proteoglycan decorin plays an important role in cell differentiation and cell migration. The cartilage-specific (V+C)(-) fibronectin isoform, in which nucleotides that normally encode the protein segments V, III(15), and I(10) are spliced out, is one of the major splice variants present in cartilage matrices. Full-length and truncated cDNA constructs were used to express recombinant versions of fibronectin.

Quantitative genetics of traits associated with hip dysplasia in a canine pedigree constructed by mating dysplastic Labrador Retrievers with unaffected Greyhounds.

Objective: To determine the genetic influence on expression of traits associated with canine hip dysplasia.

Animals: 193 dogs from an experimental canine pedigree.

Procedure: An experimental canine pedigree was developed for linkage analysis of hip dysplasia by mating dysplastic Labrador Retrievers with nondysplastic Greyhounds.