Publications by authors named "Nancy F Winters"
J Proteome Res
December 2008
Article Synopsis
- Shotgun proteome analysis using LC-MS/MS is an effective method for identifying potential biomarkers in tissue samples.
- The study compared two techniques, strong cation exchange (SCX) and isoelectric focusing (IEF), for peptide separation before LC-MS/MS analysis and found that IEF offered better reproducibility for both small and large protein inputs.
- Although SCX identified more peptides in smaller samples, IEF consistently reached peak detection levels faster, making it the preferred option for biomarker discovery in complex proteomes.
