Contrasting long-term dynamics of antimicrobial resistance and virulence plasmids in Typhimurium from animals.

Plasmids are mobile elements that can carry genes encoding traits of clinical concern, including antimicrobial resistance (AMR) and virulence. Population-level studies of , including and , indicate that plasmids are important drivers of lineage expansions and dissemination of AMR genes. Typhimurium is the second most common cause of salmonellosis in humans and livestock in the UK and Europe.

Automated reanalysis application to assist in detecting novel gene-disease associations after genome sequencing.

Purpose: This study aimed to investigate whether a bioinformatics application can streamline genome reanalysis and yield new diagnoses for patients with rare diseases.

Methods: We developed TierUp to identify variants in new disease genes for unresolved rare disease cases recruited to the 100,000 Genomes Project, all of whom underwent genome sequencing. TierUp uses the NHS Genomic Medicine Service bioinformatics infrastructure by securely accessing case details from the Clinical Interpretation Portal application programming interface and by querying the curated PanelApp database for novel gene-disease associations.

Automated reanalysis, a novel way to diagnose an ultra-rare condition: Fibronectin-1-related spondylometaphyseal dysplasia (SMD-FN1).

We report a further case of spondylometaphyseal dysplasia - corner fracture type due to the fibronectin-1 gene (SMD-FN1) in a child originally thought to have metaphyseal chondrodysplasia-Brussels type (MCD Brussels). We highlight phenotypic differences with the SMD-FN1 published reports. This case is unique in terms of the method of molecular confirmation.

Inoculants Enhance Grain Yields of Soybean and Cowpea in Northern Ghana.

This study evaluated the symbiotic effectiveness and economic evaluation of inoculants with the objective of recommending the most effective inoculant strain for soybean and cowpea production in Northern Ghana. Field experiments were established in three locations using randomized complete block design with five blocks. A total of four treatments (Legumefix, Biofix, 100 kg N ha and uninoculated control for soybean and BR 3267, BR 3262, 100 kg N ha and uninoculated control for cowpea) were applied.