Preparation of pH sensitive bacteriostatic W/O/W emulsion microcapsules.

This experiment was done to study the zeolite molecular sieve as a drug-binding effector, the non-antibiotic drug potassium diformate uniformly disperse in the internal aqueous phase of the 'egg box' structure formed by pectin-calcium ions. With oil phase as the intermediate phase and Xanthan gum Chitosan as the external water phase, the W/O/W type sustained release bacteriostatic microcapsules with pH response were prepared and characterized by Fourier transform infrared, thermogravimetric, SEM, and TEM. It can be obtained through characterization experiments that the inner water phase, oil phase, and outer water phase were formed by observation, and W/O/W emulsion microcapsules were obtained and the bacteriostasis effect of microcapsules was verified by bacteriostasis experiment.

Preparation and properties of environmentally benign waterborne polyurethane composites from sodium-alginate-modified nano calcium carbonate.

Well-dispersed inorganic nanoparticles in organic polymers are critical in the preparation of high-performance nanocomposites. This study prepared a series of waterborne polyurethane (WPU)/calcium carbonate nanocomposites using the solution blending method. Next, FT-IR, TG-DTG and XRD tests were carried out to confirm that the biopolymer sodium alginate (SA) was successfully encapsulated on the surface of the calcium carbonate nanoparticles, and that SA achieved satisfactory surface modification of the calcium carbonate nanoparticles.

Construction of pH-sensitive sodium alginates/sodium carboxymethyl cellulose/zeolite P composite hydrogel microspheres loaded with potassium diformate.

As a substitute for feed antibiotics, potassium diformate (KDF) can effectively inhibit bacterial overgrowth in the gastrointestinal tract. To avoid the sudden release of KDF in the stomach, this article proposes a new controlled drug delivery system for controlled drug release. In this system, P-type zeolite molecular sieve (Zeolite P) and drug KDF are combined and embedded into the hydrogel microspheres of sodium alginate (ALG) and sodium carboxymethyl cellulose (CMC).

Two-step hydrothermal synthesis and conversion mechanism of zeolite X from stellerite zeolite.

We investigated the conversion mechanism of stellerite zeolite to zeolite X under two-step hydrothermal conditions. To elucidate the conversion mechanism, solid products were separated from the mixtures at different crystallization times and characterized by XRD, FESEM, FT-IR, Raman, solid-state NMR, XRF, and TEM. The results indicate that in this reaction process, the Si, Al, and Na in the gel solid phases were continuously dissolved and transformed into the gel-liquid-phase.

Adsorption of As(V) from Aqueous Solution on Chitosan-Modified Diatomite.

A novel chitosan (CS)-modified diatomite (Dt) was prepared by a simple mixture in the mass ratio to remove As(Ⅴ) from aqueous solution in this research. The CS-modified Dt adsorbent was characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRD) analysis. The parameters to influence the adsorption of As(Ⅴ) ion were studied under such conditions as kinetics, adsorption isotherm, and pH effect.

Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge.

A Gram-positive, strictly anaerobic, rod-shaped bacterium, designated YB-7, was isolated from activated sludge of an anaerobic baffled reactor pond in Weizhou terminal wastewater treatment plant, Beihai, Guangxi, China. Strain YB-7 grew at pH 5.0-12.

Brain contusion as the main risk factor of memory or emotional complaints in chronic complicated mild traumatic brain injury.

Objective: To investigate the risk factors for memory or emotional complaints in patients with complicated mild traumatic brain injury (mTBI).

Methods: Retrospective analysis of medical records was conducted by physicians in a teaching hospital in Southern Taiwan, and complicated mTBI had been identified by means of computed tomography. Psychological complaints, including problems with memory and emotions, were collected by structured telephone interviews, 10-15 minutes long, and were held with subjects who agreed to participate in our study.

[Soluble expression, purification and bioactivity of recombinant human Era protein].

Aim: To prepare a soluble human Era(hEra) protein and to measure its bioactivity.

Methods: Human era cDNA gene from pUC19 plasmid was subcloned into the expression plasmid pMAL-p2x. pMAL-hEra was transducted to E.

[Construction of shRNA expression vector pWH1 and its function in silencing the HIF1 gene].

Aim: To construct the expression vector of small hairpin RNA(shRNA) and to test its efficacy in silencing the Hypoxia-inducible Factor-1 (HIF1) gene.

Methods: The H1 gene promoter was amplified from the genome of the human blood cells by PCR. Then the promoter was cloned into the pEGFP-C1 vector digested with the restriction enzyme.

Over-expression and siRNA of a novel environmental lipopolysaccharide-responding gene on the cell cycle of the human hepatoma-derived cell line HepG2.

Lipopolysaccharide (LPS) is the toxic determinant for Gram-negative bacterium infection. The individual response to LPS was related to its gene background. It is necessary to identify new molecules and signaling transduction pathways about LPS.

Expression and regulation of the yggG gene of Escherichia coli.

Our previous study indicated that Era, a membrane-associated GTPase essential for the survival of Escherichia coli, binds with the product of the yggG gene. However, the expression, regulation, and function of the yggG gene have not been established. In this study, the transcript of the yggG gene was determined by analysis of the 5'-end of the yggG mRNA using 5'-RACE (5'-rapid amplification of cDNA ends) method.

Up-regulation of yggG promotes the survival of Escherichia coli cells containing Era-1 mutant protein.

Era is a highly conserved GTPase essential for bacterial growth. Using a digoxigenin-labeled Era protein to screen a phage expression library of Escherichia coli genomic DNA, yggG, a gene that encodes a putative zinc metalloprotease was isolated and characterized. The deduced amino acid sequence of YggG showed high degrees of similarity to some reported heat shock proteins.

[Expression of mouse lipopolysaccharide response protein LRP in E. coli and preparation of rabbit anti-mLRP serum].

Aim: To express mouse lipopolysaccharide response protein (mLRP) and prepare rabbit anti-mLRP serum.

Methods: The predicted mouse lrp cDNA sequence was obtained by splicing homologous ESTs by comparing human lrp cDNA with mouse ESTs. Then the primers were designed.

[Expression of a candidate human Era binding protein A19 and the preparation of its polyclonal antibody].

Aim: To express a candidate hEra binding protein A19 in Escherichia coli and to prepare anti-A19 antibody.

Methods: A19 gene was amplified by PCR from the plasmid containing A19 gene and was cloned into the expression vector pGEX-4T3 which was then transformed into E.coli.

[Preparation of anti-Red antisera and its subcellular localization].

Aim: To prepare rabbit anti-Red antisera.

Methods: The bet, exo and gam genes of lambda phage were amplified by PCR from genomic DNA and cloned into the expression vector pDH2, respectively. Red proteins were induced to express at 42 degrees C.

[Expression and purification of two kinds of alternative splicing mouse Era].

Aim: To express and purify two alternative splicing mouse Era proteins and detect whether anti-human Era antibody can be used in the study of mouse Era proteins.

Methods: Two fusion protein expression vectors, pMAL-meraW and pMAL-meraS, were constructed, then the MBP-mEra proteins were expressed in E. coli.

[The expression of truncated YggG protein and preparation of its polyclonal antibody].

Aim: To express truncated YggG protein (TYP) in Escherichia coli and to prepare anti-TYPE antibody.

Methods: Truncated yggg gene was amplified by PCR from the plasmid containing full length yggg gene and was cloned into the expression vector pDH2. The expression of TYP was achieved by thermal induction.

[High density fed-batch culture of Escherichia coli DH5 alpha/pDH-B2m with DO feed-back control of nutrient feeding].

Optimization of cultivation condition of recombinant E. coli DH5 alpha/pDH-B2m and the condition suitable for expression of recombinant mature peptide of human bone morphogenetic protein-2 was carried out in 500 mL shaking flasks and then transferred to NBS Bioflo IV, a 20 L DO feed-back fed-batch culture system, to obtain rhBMP-2. The results indicate that keeping dissolved oxygen at 40% and controlling nutrient feeding rate with DO feed back strategy can obtain theoretically 3.

Cloning and Optimized Expression of Human Angiogenin in E.coli.

Angiogenin(ANG) is an important factor of angiogenesis during different stage of tumor development and exists widely in various tumors. To study the biological funcption and find the antagonistic drugs of angiogenin, the angiogenin was allowed to be expressed by E.coli.