Publications by authors named "Nan-Yun Tang"
Cloning of GAP Gene from K. fragilis.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 1998
The K. fragilis CBS 397 gene library was screened with a GAP probe, which was designed according to the homology with S. cerevisiae, K.
View Article and Find Full Text PDFCloning of the K. fragilis PDI Gene.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 1997
The K. fragilis CBS 397 gene library was screened by the PDI probe, which was designed according to the homology with the S. cerevisiae PDI gene.
View Article and Find Full Text PDFThe Mutation of mRNA Translation Initiation Region Influences Gene Translation.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 1997
In order to investigate the relationship between the structure of the mRNA translation initiation region (TIR) and gene expression, We mutated multiple sites of the 5' end of IFN-alpha8 and GM-CSF genes by site-directed mutagenesis without changing their amino acid sequences. SDS-PAGE showed that the protein products of mutated genes increased greatly in recombinant clones, as compared with their native genes. RNA dot blot revealed that the difference of their corresponding amount of mRNA transcribed between the native and the mutated genes was negligible.
View Article and Find Full Text PDFThe Construction and Application of the Multi-copy Integration Vector in K. lactis.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 1996
Article Synopsis
- The vector pIRK was created using a fragment of rDNA from Kluyveromyces lactis and included the URA3 gene from Saccharomyces cerevisiae for selection.
- In K. lactis transformants, pIRK had an average of 120 copies per cell and demonstrated high stability after 50 generations, with all integrations found on chromosome IV.
- The LAC4 gene from K. fragilis was expressed using pIRK, resulting in beta-galactosidase production that was 8.6 times greater than the wild type strain under the same conditions.