Exploring sp. M21F004 for Biocontrol of Bacterial and Fungal Phytopathogens.

This study explores the biocontrol potential of sp. M21F004, a lactic acid bacteria (LAB) isolated from marine environments, against several bacterial and fungal phytopathogens. Out of 50 marine bacterial isolates, sp.

Development of a new broad-spectrum microencapsulation-based spray drying formulation of subsp. IMBL-B9 for the control of moths.

Certain (Bt) strains such as Bt subsp. and Bt subsp. have been widely used for pest management in agricultural practices.

and antimicrobial activity of the fungal metabolite toluquinol against phytopathogenic bacteria.

Introduction: Bacterial plant diseases cause tremendous economic losses worldwide. However, a few effective and sustainable control methods are currently available. To discover novel and effective management approaches, we screened marine fungi for their antibacterial activity against phytopathogenic bacteria and .

Root-Knot Nematode () Control Using a Combination of WiKim0090 and Copper Sulfate.

Lactic acid bacteria (LAB) exert antagonistic activity against root-knot nematodes, mainly by producing organic acids via carbohydrate fermentation. However, they have not yet been used for root-knot nematode () control owing to a lack of economic feasibility and effectiveness. In this study, we aimed to isolate organic acid-producing LAB from kimchi (Korean traditional fermented cabbage) and evaluated their nematicidal activity.

sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases.

Bacteria and fungi are major phytopathogens which substantially affect global agricultural productivity. In the present study, sp. AN090126, isolated from agricultural suppressive soil in Korea, showed broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi.

Identification, Characterization, and Efficacy Evaluation of Bacillus velezensis for Shot-Hole Disease Biocontrol in Flowering Cherry.

Though information exists regarding the pathogenesis of the shot-hole disease (SH) in flowering cherry (FC), there has been a lack of research focusing on SH management. Therefore, here, we investigated the inhibitory activities of antagonistic bacteria against SH pathogens both in vitro and in vivo as well as their biochemical characteristics and bioactive compounds. Two biosurfactant-producing bacterial antagonists, identified as Bacillus velezensis strains JCK-1618 and JCK-1696, exhibited the best effects against the growth of both bacterial and fungal SH pathogens in vitro through their cell-free culture filtrates (CFCFs).

Nematicidal Activity of Cyclopiazonic Acid Derived From Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process.

Among 200 fungal strains isolated from the soil, only one culture filtrate of JCK-4087 showed strong nematicidal activity against . The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of , which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity.

Optimization of -Mediated Transformation of EL000614, an Endolichenic Fungus Producing Grammicin.

An endolichenic fungus EL000614 produces grammicin, a potent nematicidal pyrone derivative that can serve as a new control option for root-knot nematodes. We optimized an -mediated transformation (ATMT) protocol for to support genetic studies. Transformants were successfully generated after co-cultivation of homogenized young mycelia of with strain AGL-1 carrying a binary vector that contains the bacterial hygromycin B phosphotransferase () gene and the gene in T-DNA.

sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms.

Plant bacterial and fungal diseases cause significant agricultural losses and need to be controlled. Beneficial bacteria are promising candidates for controlling these diseases. In this study, sp.

Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in KCTC 13121BP against .

Grammicin, a polyketide metabolite produced by the endolichenic fungus KCTC 13121BP, shows strong nematicidal activity against This study was performed to elucidate the grammicin biosynthesis pathway of KCTC 13121BP and to examine the nematicidal activity of the biosynthesis intermediates and derivatives against . Two grammicin biosynthesis intermediates were isolated from a T-DNA insertion transformant (strain TR-74) of KCTC 13121BP and identified as 2-(hydroxymethyl)cyclohexa-2,5-diene-1,4-dione (compound ) and 2,5-dihydroxybenzaldehyde (compound ), which were also reported to be intermediates in the biosynthesis pathway of patulin, an isomer of grammicin. This indicates that the grammicin biosynthesis pathway overlaps almost with that of patulin, except for the last few steps.

Draft Genome Sequence of EL000614, a Strain Producing Grammicin, a Potent Nematicidal Compound.

An endolichenic fungus, strain EL000614, showed strong nematicidal effects against plant pathogenic nematode, by producing grammicin. We report genome assembly of EL000614 comprised of 25 scaffolds with a total length of 54.73 Mb, N50 of 4.

First Report of Shot-Hole on Flowering Cherry Caused by and pv. .

Shot-hole disease (SH) is one of the most common and important diseases affecting flowering cherry (FC; Matsumura; Somei-yoshino) trees in South Korea every year, resulting in premature defoliation and reduced flowering in the following year. However, pathogens associated with the disease remain unknown, which has rendered disease management challenging. Here, the pathogens associated with SH, their biochemical characteristics, and their host range were elucidated.

and Antibacterial Activity of Serratamid, a Novel Peptide-Polyketide Antibiotic Isolated from C1, against Phytopathogenic Bacteria.

A new hybrid non-ribosomal peptide-polyketide antibiotic (serratamid) for phytoprotection was isolated from the ethyl acetate layer of tryptic soy agar culture of the soil bacterium C1 through bioassay-guided fractionation. Its chemical structure was elucidated using instrumental analyses, such as mass and nuclear magnetic resonance spectrometry. Serratamid showed antibacterial activity against 15 phytopathogenic bacteria, with minimum inhibitory concentration (MIC) values ranging from 0.

Deciphering the Relationship Between Cycloheximides Structures and Their Different Biological Activities.

species are the most important sources of antibacterial, antifungal, and phytotoxic metabolites. In this study, cycloheximide (CH) and acetoxycycloheximide (ACH) were isolated from the fermentation broth of sp. JCK-6092.

First Report of Associated with Leaf Spot on Flowering Cherry in South Korea.

Flowering cherry (FC, Matsumura; Somei-yoshino cherry) is an ornamental tree, planted across South Korea and producing stunning flowers in spring. The seasonal blooms are annually celebrated during cherry blossom festivals in many locations across the country. The leaf spot disease is among the most common and important diseases affecting FC trees every year, resulting in premature defoliation and reduced flowering of cherry blossoms in the following year.

Biological Control of Tomato Bacterial Wilt, Kimchi Cabbage Soft Rot, and Red Pepper Bacterial Leaf Spot Using JCK-5075.

The over and repeated use of chemical bactericides to control plant bacterial diseases has resulted in unwanted effects, such as environmental pollution, residual toxicity, and resistance buildup in bacterial pathogens. Many previous studies have aimed to develop biological control agents to replace chemical bactericides. In this study, the antibacterial efficacy of the fermentation broth of JCK-5075 and its antibacterial compounds were evaluated against plant pathogenic bacteria, using both and bioassays.

Corrigendum: The Hsp90 Inhibitor, Monorden, Is a Promising Lead Compound for the Development of Novel Fungicides.

[This corrects the article DOI: 10.3389/fpls.2020.

The Hsp90 Inhibitor, Monorden, Is a Promising Lead Compound for the Development of Novel Fungicides.

Endophytic fungi are great resources for the identification of useful natural products such as antimicrobial agents. In this study, we performed the antifungal screening of various plant endophytic fungi against the dollar spot pathogen and finally selected sp. JS-0112 as a potential biocontrol agent.

Structure and antifungal activity of pelgipeptins from Paenibacillus elgii against phytopathogenic fungi.

Paenibacillus elgii JCK1400 shows strong antifungal activity against various plant pathogenic fungi in vitro, but little is known about its mode of action. Four antifungal lipopeptides were isolated from P. elgii JCK1400 using bioassay-directed fractionation.

Biological control of tomato bacterial wilt by oxydifficidin and difficidin-producing Bacillus methylotrophicus DR-08.

Bacillus methylotrophicus DR-08 exhibited strong antibacterial activity against Ralstonia solanacearum, a causal agent of tomato bacterial wilt. This study aimed to identify the antibacterial metabolites and evaluate the efficacy of the strain as a biocontrol agent for tomato bacterial wilt. A butanol extract of the DR-08 broth culture completely inhibited the growth of 14 phytopathogenic bacteria with minimum inhibitory concentration (MIC) values of 1.

Biological Control of Root-Knot Nematodes by Organic Acid-Producing WiKim0069 Isolated from Kimchi.

Root-knot nematodes (RKNs) are among the most destructive plant-parasites worldwide, and RKN control has been attempted mainly using chemical nematicides. However, these chemical nematicides have negative effects on humans and the environment, thus necessitating the search for eco-friendly alternative RKN control methods. Here, we screened nematicidal lactic acid bacteria (LAB) isolated from kimchi and evaluated their efficacy as biocontrol agents against RKNs.

Draft Genome Sequence of JS-1675, an Endophytic Fungus from .

The fungus strain JS-1675 has been reported to exert antimicrobial effects against various plant-pathogenic bacteria and fungi. Here, we report the draft genome sequence of for the first time. The assembly comprises 48,177,783 bp with 18 scaffolds.

Characterization of DA12 Showing Potent Antifungal Activity against Mycotoxigenic Species.

In an attempt to develop a biological control agent against mycotoxigenic species, we isolated strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic , , , and both and (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including , , , , , and .