Publications by authors named "Namratha Tarigopula"
Background: The increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance and control. Genomic surveillance of dengue virus not only provides important insights into the emergence and spread of genetically diverse serotypes and genotypes, but it is also critical to monitor the effectiveness of newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome sequencing of all four dengue virus serotypes.
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- The study investigates the use of next-generation sequencing to identify genetic mutations related to antibiotic resistance in clinical samples, particularly focusing on the bacterium that is challenging to culture and requires molecular methods for detection.* -
- In the analysis of 98 specimens, the researchers found mutations in 94 samples, totaling 469 single nucleotide polymorphisms (SNPs), which could help create comprehensive drug resistance profiles for macrolides and fluoroquinolones within a typical turnaround time of 3 days.* -
- The proposed method promises to enhance the understanding and diagnosis of antibiotic resistance mechanisms, paving the way for its potential adoption in clinical laboratories after thorough validation.*
Background: The increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance and control. Genomic surveillance of dengue virus not only provides important insights into the emergence and spread of genetically diverse serotypes and genotypes, but it is also critical to monitor the effectiveness of newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome sequencing of all four dengue virus serotypes.
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