Digital contrast sensitivity chart with varying visual acuity: Development and validation.

Background: Contrast sensitivity (CS) evaluation is very much necessary for the diagnosis and management of eye conditions. Several disadvantages seen in the traditionally used Pelli-Robson (PR) chart persuaded us to develop a novel digital device for the evaluation of CS at various levels of visual acuity (VA) for early detection of deteriorating vision.

Materials And Methods: The new chart was constructed on the Debian software.

The impact of COVID-19 on compliance to amblyopia treatment in a tertiary eye care center.

Purpose: To investigate the impact of the coronavirus disease 2019 (COVID-19) outbreak on compliance to amblyopia therapy from the perspective of parents of amblyopic children.

Methods: The participants for the qualitative study were enrolled from the medical records of a tertiary eye care center during the COVID-19 pandemic. The trained researcher conducted telephonic interviews consisting of validated 15 open-ended questions for 15 min.

Phytochemical profiling of leaf extracts: GC-MS, LC-MS analysis and comparative evaluation of antimicrobial, antioxidant & cytotoxic effects.

The present study evaluates the phytochemical content and biological activities of eight leaf extracts obtained using four solvents of varying polarity and two extraction methods. GC-HRMS analysis of the hexane extract predicted presence of phytol, 22-tritetracontanone, and 6,9,12-octadecatrienoic acid, phenyl methyl ester, (ZZZ)-as major compounds, and ethyl acetate extract was predicted to contain phytol. LC-HRMS analysis of methanolic extract exhibited the presence of 8',10'-dihydroxydihydroergotamine, Khayanthone, Galactonic acid, Calotropin, and 26,26,26,27,27,27-hexafluoro-1alpha,24-dihydroxy vitamin D3 as significant fractions.

Isolation, identification and biological evaluation of phytochemicals from : a medicinal plant endemic to Western Ghats of India.

The present investigation reports evaluation of phytochemical content, antimicrobial, antioxidant, antidiabetic and anticancer properties of the methanolic crude extracts prepared from leaves. Qualitative evaluation showed the presence of carbohydrates, reducing sugars, alkaloids, phenols, flavonoids, cardiac glycosides, steroids, phytosterols, terpenoids, diterpenoids and coumarins. Quantitative estimation for phenols, flavonoids and alkaloids indicated total phenolic content 11.

A plastid-localized bona fide geranylgeranyl diphosphate synthase plays a necessary role in monoterpene indole alkaloid biosynthesis in Catharanthus roseus.

In plants, geranylgeranyl diphosphate (GGPP, C ) synthesized by GGPP synthase (GGPPS) serves as precursor for vital metabolic branches including specialized metabolites. Here, we report the characterization of a GGPPS (CrGGPPS2) from the Madagascar periwinkle (Catharanthus roseus) and demonstrate its role in monoterpene (C )-indole alkaloids (MIA) biosynthesis. The expression of CrGGPPS2 was not induced in response to methyl jasmonate (MeJA), and was similar to the gene encoding type-I protein geranylgeranyltransferase_β subunit (CrPGGT-I_β), which modulates MIA formation in C.

Polyamine Induction in Postharvest Banana Fruits in Response to NO Donor SNP Occurs via l-Arginine Mediated Pathway and Not via Competitive Diversion of S-Adenosyl-l-Methionine.

Nitric oxide (NO) is known to antagonize ethylene by various mechanisms; one of such mechanisms is reducing ethylene levels by competitive action on S-adenosyl-L-methionine (SAM)-a common precursor for both ethylene and polyamines (PAs) biosynthesis. In order to investigate whether this mechanism of SAM pool diversion by NO occur towards PAs biosynthesis in banana, we studied the effect of NO on alterations in the levels of PAs, which in turn modulate ethylene levels during ripening. In response to NO donor sodium nitroprusside (SNP) treatment, all three major PAs viz.