Studying the characteristics of nanobody CDR regions based on sequence analysis in combination with 3D structures.

Background: Single-domain antibodies or nanobodies have recently attracted much attention in research and applications because of their great potential and advantage over conventional antibodies. However, isolation of candidate nanobodies in the lab has been costly and time-consuming. Screening of leading nanobody candidates through synthetic libraries is a promising alternative, but it requires prior knowledge to control the diversity of the complementarity-determining regions (CDRs) while still maintaining functionality.

A New Pathway for the Preparation of Pyrano[2,3-]pyrazoles and molecular Docking as Inhibitors of p38 MAP Kinase.

We report a new pathway to synthesize pyrano[2,3-]pyrazoles and their binding mode to p38 MAP kinase. Pyrano[2,3-]pyrazole derivatives have been prepared through a four-component reaction of benzyl alcohols, ethyl acetoacetate, phenylhydrazine, and malononitrile in the presence of sulfonated amorphous carbon and eosin Y as catalysts. All products were characterized by melting point, H and C NMR, and HRMS (ESI).

A Novel Method to Predict Highly Expressed Genes Based on Radius Clustering and Relative Synonymous Codon Usage.

Recombinant proteins play an important role in many aspects of life and have generated a huge income, notably in the industrial enzyme business. A gene is introduced into a vector and expressed in a host organism-for example, E. coli-to obtain a high productivity of target protein.